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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F02%3A00028886%21RIV10-GA0-14310___
rdf:type
n17:Vysledek skos:Concept
dcterms:description
Modifications of natural DNA by the dinuclear platinum(II) organometallic complex [{Pt(Me)Cl(Me2SO)}2(-N-N)] [where N-N=H2N(CH2)6NH2] (ORGANObisPt) were studied by methods of molecular biophysics. These methods include DNA binding studies using atomic absorption spectrophotometry, HPLC analysis of enzymatically digested DNA, interstrand cross-linking employing gel electrophoresis under denaturing conditions, DNA unwinding studied by gel electrophoresis, mapping of DNA adducts by transcription assay, DNA melting curves measured by absorption spectrophotometry and conformational analysis of platinated DNA by differential pulse polarography. The results indicate that the complex ORGANObisPt binds irreversibly to DNA. Its DNA binding mode is, however, different from that of the formally equivalent [{cis-PtCl(NH3)2}2(-N-N)] (1,1/c,c), which exhibits antitumor activity including that in the tumor cells resistant to cisplatin. Modifications of natural DNA by the dinuclear platinum(II) organometallic complex [{Pt(Me)Cl(Me2SO)}2(-N-N)] [where N-N=H2N(CH2)6NH2] (ORGANObisPt) were studied by methods of molecular biophysics. These methods include DNA binding studies using atomic absorption spectrophotometry, HPLC analysis of enzymatically digested DNA, interstrand cross-linking employing gel electrophoresis under denaturing conditions, DNA unwinding studied by gel electrophoresis, mapping of DNA adducts by transcription assay, DNA melting curves measured by absorption spectrophotometry and conformational analysis of platinated DNA by differential pulse polarography. The results indicate that the complex ORGANObisPt binds irreversibly to DNA. Its DNA binding mode is, however, different from that of the formally equivalent [{cis-PtCl(NH3)2}2(-N-N)] (1,1/c,c), which exhibits antitumor activity including that in the tumor cells resistant to cisplatin.
dcterms:title
Biophysical analysis of natural, double-helical DNA modified by a dinuclear platinum(II) organometallic compound in a cell-free medium Biophysical analysis of natural, double-helical DNA modified by a dinuclear platinum(II) organometallic compound in a cell-free medium
skos:prefLabel
Biophysical analysis of natural, double-helical DNA modified by a dinuclear platinum(II) organometallic compound in a cell-free medium Biophysical analysis of natural, double-helical DNA modified by a dinuclear platinum(II) organometallic compound in a cell-free medium
skos:notation
RIV/00216224:14310/02:00028886!RIV10-GA0-14310___
n3:aktivita
n9:P n9:Z
n3:aktivity
P(GA301/00/0556), P(GA305/02/1552), P(IAA5004101), P(NL6058), Z(AV0Z5004920)
n3:cisloPeriodika
7
n3:dodaniDat
n11:2010
n3:domaciTvurceVysledku
n12:2087960 n12:7988346 n12:4571932
n3:druhVysledku
n6:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
639728
n3:idVysledku
RIV/00216224:14310/02:00028886
n3:jazykVysledku
n19:eng
n3:klicovaSlova
DNA; Organoplatinum(II); Adducts; Cross-link; Conformation
n3:klicoveSlovo
n4:Organoplatinum%28II%29 n4:Adducts n4:Conformation n4:DNA n4:Cross-link
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[530A023C762A]
n3:nazevZdroje
Journal of Biological Inorganic Chemistry
n3:obor
n16:BO
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
6
n3:projekt
n10:GA305%2F02%2F1552 n10:IAA5004101 n10:GA301%2F00%2F0556 n10:NL6058
n3:rokUplatneniVysledku
n11:2002
n3:svazekPeriodika
7
n3:tvurceVysledku
Brabec, Viktor Monsu Scolaro, Luigi Novakova, Olga Marini Palomeque, María Victoria Romeo, Raffaello Kašpárková, Jana
n3:zamer
n18:AV0Z5004920
s:issn
0949-8257
s:numberOfPages
10
n7:organizacniJednotka
14310