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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F02%3A00007137%21RIV09-GA0-14310___
rdf:type
n4:Vysledek skos:Concept
dcterms:description
On the whole, 128 staphylococcal isolates (Staphylococcus aureus 65, Staphylococcus epidermidis 42, and Staphylococcus haemolyticus 21 strains) from patients of ten Czech hospitals were classified as susceptible or resistant to oxacillin by the standard broth dilution micro-method (using the MIC break-point of 4.0 mg/L for resistance). The MRSA-Screen test (Denka Seiken Co., Japan), a latex agglutination for the detection of penicillin binding protein PBP2a in oxacillin/methicillin-resistant staphylococci, was compared with other commonly used phenotypic susceptibility tests. The PCR detection of the mecA gene, the presence of which is equaled to oxacillin resistance, was used as a reference genotypic method. In addition, the determination of the femA gene was performed also by this procedure. All 68 isolates (S. aureus 26, S. epidermidis 21, and S. haemolyticus 21 strains), determined as oxacillin-resistant, contained the mecA gene. The femA gene was present in all these S. aureus strains. The other On the whole, 128 staphylococcal isolates (Staphylococcus aureus 65, Staphylococcus epidermidis 42, and Staphylococcus haemolyticus 21 strains) from patients of ten Czech hospitals were classified as susceptible or resistant to oxacillin by the standard broth dilution micro-method (using the MIC break-point of 4.0 mg/L for resistance). The MRSA-Screen test (Denka Seiken Co., Japan), a latex agglutination for the detection of penicillin binding protein PBP2a in oxacillin/methicillin-resistant staphylococci, was compared with other commonly used phenotypic susceptibility tests. The PCR detection of the mecA gene, the presence of which is equaled to oxacillin resistance, was used as a reference genotypic method. In addition, the determination of the femA gene was performed also by this procedure. All 68 isolates (S. aureus 26, S. epidermidis 21, and S. haemolyticus 21 strains), determined as oxacillin-resistant, contained the mecA gene. The femA gene was present in all these S. aureus strains. The other On the whole, 128 staphylococcal isolates (Staphylococcus aureus 65, Staphylococcus epidermidis 42, and Staphylococcus haemolyticus 21 strains) from patients of ten Czech hospitals were classified as susceptible or resistant to oxacillin by the standard broth dilution micro-method (using the MIC break-point of 4.0 mg/L for resistance). The MRSA-Screen test (Denka Seiken Co., Japan), a latex agglutination for the detection of penicillin binding protein PBP2a in oxacillin/methicillin-resistant staphylococci, was compared with other commonly used phenotypic susceptibility tests. The PCR detection of the mecA gene, the presence of which is equaled to oxacillin resistance, was used as a reference genotypic method. In addition, the determination of the femA gene was performed also by this procedure. All 68 isolates (S. aureus 26, S. epidermidis 21, and S. haemolyticus 21 strains), determined as oxacillin-resistant, contained the mecA gene. The femA gene was present in all these S. aureus strains. The other
dcterms:title
Comparison of MRSA-screen latex agglutination, conventional phenotypic methods and <I>mecA</I> gene detection for identification of oxacilin resistance in staphylococci Comparison of MRSA-screen latex agglutination, conventional phenotypic methods and <I>mecA</I> gene detection for identification of oxacilin resistance in staphylococci Comparison of MRSA-screen latex agglutination, conventional phenotypic methods and <I>mecA</I> gene detection for identification of oxacilin resistance in staphylococci
skos:prefLabel
Comparison of MRSA-screen latex agglutination, conventional phenotypic methods and <I>mecA</I> gene detection for identification of oxacilin resistance in staphylococci Comparison of MRSA-screen latex agglutination, conventional phenotypic methods and <I>mecA</I> gene detection for identification of oxacilin resistance in staphylococci Comparison of MRSA-screen latex agglutination, conventional phenotypic methods and <I>mecA</I> gene detection for identification of oxacilin resistance in staphylococci
skos:notation
RIV/00216224:14310/02:00007137!RIV09-GA0-14310___
n3:aktivita
n15:P n15:Z
n3:aktivity
P(GA301/02/1505), P(GA301/99/D075), Z(MSM 143100008)
n3:cisloPeriodika
6
n3:dodaniDat
n14:2009
n3:domaciTvurceVysledku
n9:9138498 n9:3440656 n9:8758298 n9:5051207
n3:druhVysledku
n16:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
641314
n3:idVysledku
RIV/00216224:14310/02:00007137
n3:jazykVysledku
n10:eng
n3:klicovaSlova
Bacterial Typing Techniques; DNA; Staphylococcus aureus; Coagulase-Negative Staphylococci; Methicillin/Oxacillin Resistance; Screen Latex Agglutination
n3:klicoveSlovo
n8:Coagulase-Negative%20Staphylococci n8:Staphylococcus%20aureus n8:Methicillin%2FOxacillin%20Resistance n8:Screen%20Latex%20Agglutination n8:DNA n8:Bacterial%20Typing%20Techniques
n3:kodStatuVydavatele
SK - Slovenská republika
n3:kontrolniKodProRIV
[7327DFA8900F]
n3:nazevZdroje
Biologia, Section: Cellular and Molecular Biology
n3:obor
n5:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
5
n3:projekt
n17:GA301%2F99%2FD075 n17:GA301%2F02%2F1505
n3:rokUplatneniVysledku
n14:2002
n3:svazekPeriodika
57
n3:tvurceVysledku
Doškař, Jiří Rosypal, Stanislav Kolář, Milan Pantůček, Roman Hájek, Václav
n3:wos
000180726700011
n3:zamer
n6:MSM%20143100008
s:issn
1335-6399
s:numberOfPages
10
n19:organizacniJednotka
14310