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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F02%3A00007098%21RIV08-MSM-14310___
rdf:type
skos:Concept n10:Vysledek
dcterms:description
The genus Armillaria belongs to basidiomycetes and has been known to induce root rot disease and to cause extensive economic losses to a forest crop. More than 40 isolates containing six species of Armillaria from the collections have been analyzed (A. borealis, A. cepistipes, A. gallica, A. mellea, A. ostoyae and A. tabescens). We have used the internal transcribed spacer (ITS), which is highly polymorphic and provides useful tool for taxonomic studies thanks to highly conserved sequences of these region. The resolution achieved on the HPLC system was comparable or better than that achieved by electrophoresis and HPLC has better performance in quantitation. Moreover, the restriction analysis of the isolates using HPLC allowed to clearly differentiate some heterozygots from homozygots. The genus Armillaria belongs to basidiomycetes and has been known to induce root rot disease and to cause extensive economic losses to a forest crop. More than 40 isolates containing six species of Armillaria from the collections have been analyzed (A. borealis, A. cepistipes, A. gallica, A. mellea, A. ostoyae and A. tabescens). We have used the internal transcribed spacer (ITS), which is highly polymorphic and provides useful tool for taxonomic studies thanks to highly conserved sequences of these region. The resolution achieved on the HPLC system was comparable or better than that achieved by electrophoresis and HPLC has better performance in quantitation. Moreover, the restriction analysis of the isolates using HPLC allowed to clearly differentiate some heterozygots from homozygots. Diskriminace Armillaria metodami mol. biologie a HPLC
dcterms:title
Discrimination of Armillaria species based on molecular biological methods and HPLC. Diskriminace Armillaria metodami mol. biologie a HPLC Discrimination of Armillaria species based on molecular biological methods and HPLC.
skos:prefLabel
Diskriminace Armillaria metodami mol. biologie a HPLC Discrimination of Armillaria species based on molecular biological methods and HPLC. Discrimination of Armillaria species based on molecular biological methods and HPLC.
skos:notation
RIV/00216224:14310/02:00007098!RIV08-MSM-14310___
n3:strany
111
n3:aktivita
n8:Z n8:P
n3:aktivity
P(NF5991), P(NF6520), Z(MSM 143100005)
n3:dodaniDat
n19:2008
n3:domaciTvurceVysledku
n15:9713050 n15:3881652 n15:9217088
n3:druhVysledku
n16:D
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
643311
n3:idVysledku
RIV/00216224:14310/02:00007098
n3:jazykVysledku
n14:eng
n3:klicovaSlova
Armillaria; PCR; discrimination; HPLC
n3:klicoveSlovo
n9:discrimination n9:HPLC n9:PCR n9:Armillaria
n3:kontrolniKodProRIV
[1E7E0DC9E637]
n3:mistoKonaniAkce
22-25 May 2002, Kusadasi, Pine Bay, Turkey
n3:mistoVydani
Kusadasi, Turkey
n3:nazevZdroje
Book of Abstracts of 7th Meeting of WG
n3:obor
n11:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:projekt
n21:NF6520 n21:NF5991
n3:rokUplatneniVysledku
n19:2002
n3:tvurceVysledku
Mikeš, Vladimír Lochman, Jan Šerý, Omar Jankovský, Libor
n3:typAkce
n12:WRD
n3:zahajeniAkce
2002-01-01+01:00
n3:zamer
n13:MSM%20143100005
s:numberOfPages
1
n5:hasPublisher
IOBC, OILB
n18:organizacniJednotka
14310