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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F02%3A00006665%21RIV08-GA0-14310___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
Plasmid pBR322 DNA was purified by gel permeation chromatography. Recovered plasmid DNA was free of RNA and retained its biological activity. The biological activity of purified plasmid DNA was tested by electrotransformation into bacterial cells. DNA fragments of lambda DNA and lambda DNA were separated on column for gel permeation chromatography. Small fragments were eluted in void volume; large fragments and lambda DNA were separated in reversed order than expected according to GPC and according to the slalom chromatography mechanisms. Plasmid pBR322 DNA was purified by gel permeation chromatography. Recovered plasmid DNA was free of RNA and retained its biological activity. The biological activity of purified plasmid DNA was tested by electrotransformation into bacterial cells. DNA fragments of lambda DNA and lambda DNA were separated on column for gel permeation chromatography. Small fragments were eluted in void volume; large fragments and lambda DNA were separated in reversed order than expected according to GPC and according to the slalom chromatography mechanisms. Plasmid pBR322 DNA was purified by gel permeation chromatography. Recovered plasmid DNA was free of RNA and retained its biological activity. The biological activity of purified plasmid DNA was tested by electrotransformation into bacterial cells. DNA fragments of lambda DNA and lambda DNA were separated on column for gel permeation chromatography. Small fragments were eluted in void volume; large fragments and lambda DNA were separated in reversed order than expected according to GPC and according to the slalom chromatography mechanisms.
dcterms:title
Chromatographic purification and separation of plasmid and lambda phage DNAs Chromatographic purification and separation of plasmid and lambda phage DNAs Chromatographic purification and separation of plasmid and lambda phage DNAs
skos:prefLabel
Chromatographic purification and separation of plasmid and lambda phage DNAs Chromatographic purification and separation of plasmid and lambda phage DNAs Chromatographic purification and separation of plasmid and lambda phage DNAs
skos:notation
RIV/00216224:14310/02:00006665!RIV08-GA0-14310___
n3:strany
53-53
n3:aktivita
n18:P
n3:aktivity
P(GA203/00/1339)
n3:dodaniDat
n14:2008
n3:domaciTvurceVysledku
n13:2412594 n13:4977459 n13:5881684
n3:druhVysledku
n4:D
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
640859
n3:idVysledku
RIV/00216224:14310/02:00006665
n3:jazykVysledku
n8:eng
n3:klicovaSlova
Plasmid and phage DNAs; purification; chromatography
n3:klicoveSlovo
n7:Plasmid%20and%20phage%20DNAs n7:chromatography n7:purification
n3:kontrolniKodProRIV
[FF2092D8F13D]
n3:mistoKonaniAkce
November, 10-13, 2002, Heidelberg, Germany
n3:mistoVydani
Heidelberg, Germany
n3:nazevZdroje
22nd International Symposium on the Separation of Proteins, Peptides and Polynucleotides
n3:obor
n9:EI
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n16:GA203%2F00%2F1339
n3:rokUplatneniVysledku
n14:2002
n3:tvurceVysledku
Rittich, Bohuslav Španová, Alena Skalníková, Magdalena
n3:typAkce
n12:WRD
n3:zahajeniAkce
2002-01-01+01:00
s:numberOfPages
1
n20:hasPublisher
Dechema
n17:organizacniJednotka
14310