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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F02%3A00006633%21RIV09-GA0-14310___
rdf:type
skos:Concept n21:Vysledek
dcterms:description
Background.Bacteriophages play an important part in gene transfer and gene expression in S.aureus strains and thus also in their virulence and antibiotic resistance.A set of primers for multiplex PCR was designed for identification of prophages of serogroups A,B,F and L in lysogenic and polylysogenic S.aureus strains. Methods.In the genomes of International Typing Set bacteriophages,the sequences specific for both the phage species and serogroups A (ö 3A),B (ö 53),F (ö 77)and L (ö 187)were estimated.The designed primers amplify specific genomic sequences of sizes characteristic of each of the phage species. The multiplex PCR specificity was verified on DNAs of 26 bacteriophages,9 lysogenized and 2 prophageless S.aureus strains. Results.One to three prophages of different serogroups were identified unambiguously in each of 40 clinical strains including 25 MRSA strains.Detectability of prophages by multiplex PCR is comparable with that by phage-specific probes prepared in our laboratory (Doskar et al.,2 Background.Bacteriophages play an important part in gene transfer and gene expression in S.aureus strains and thus also in their virulence and antibiotic resistance.A set of primers for multiplex PCR was designed for identification of prophages of serogroups A,B,F and L in lysogenic and polylysogenic S.aureus strains. Methods.In the genomes of International Typing Set bacteriophages,the sequences specific for both the phage species and serogroups A (ö 3A),B (ö 53),F (ö 77)and L (ö 187)were estimated.The designed primers amplify specific genomic sequences of sizes characteristic of each of the phage species. The multiplex PCR specificity was verified on DNAs of 26 bacteriophages,9 lysogenized and 2 prophageless S.aureus strains. Results.One to three prophages of different serogroups were identified unambiguously in each of 40 clinical strains including 25 MRSA strains.Detectability of prophages by multiplex PCR is comparable with that by phage-specific probes prepared in our laboratory (Doskar et al.,2 Background.Bacteriophages play an important part in gene transfer and gene expression in S.aureus strains and thus also in their virulence and antibiotic resistance.A set of primers for multiplex PCR was designed for identification of prophages of serogroups A,B,F and L in lysogenic and polylysogenic S.aureus strains. Methods.In the genomes of International Typing Set bacteriophages,the sequences specific for both the phage species and serogroups A (ö 3A),B (ö 53),F (ö 77)and L (ö 187)were estimated.The designed primers amplify specific genomic sequences of sizes characteristic of each of the phage species. The multiplex PCR specificity was verified on DNAs of 26 bacteriophages,9 lysogenized and 2 prophageless S.aureus strains. Results.One to three prophages of different serogroups were identified unambiguously in each of 40 clinical strains including 25 MRSA strains.Detectability of prophages by multiplex PCR is comparable with that by phage-specific probes prepared in our laboratory (Doskar et al.,2
dcterms:title
Multiplex PCR for detection of <I>Staphylococcus aureus</I> prophages Multiplex PCR for detection of <I>Staphylococcus aureus</I> prophages Multiplex PCR for detection of <I>Staphylococcus aureus</I> prophages
skos:prefLabel
Multiplex PCR for detection of <I>Staphylococcus aureus</I> prophages Multiplex PCR for detection of <I>Staphylococcus aureus</I> prophages Multiplex PCR for detection of <I>Staphylococcus aureus</I> prophages
skos:notation
RIV/00216224:14310/02:00006633!RIV09-GA0-14310___
n6:aktivita
n20:Z n20:P
n6:aktivity
P(GA301/02/1505), Z(MSM 143100008)
n6:dodaniDat
n9:2009
n6:domaciTvurceVysledku
n14:4186672 n14:3440656 n14:7675011 n14:5051207 n14:3647706
n6:druhVysledku
n12:D
n6:duvernostUdaju
n18:S
n6:entitaPredkladatele
n16:predkladatel
n6:idSjednocenehoVysledku
654638
n6:idVysledku
RIV/00216224:14310/02:00006633
n6:jazykVysledku
n7:eng
n6:klicovaSlova
Bacterial Typing Techniques; DNA; Bacteriophages; Staphylococcus aureus; Lysogeny; Multiplex PCR; Prophage detection
n6:klicoveSlovo
n10:Staphylococcus%20aureus n10:Lysogeny n10:Multiplex%20PCR n10:DNA n10:Bacteriophages n10:Bacterial%20Typing%20Techniques n10:Prophage%20detection
n6:kontrolniKodProRIV
[BF1192E31075]
n6:mistoKonaniAkce
October 16-19, 2002
n6:mistoVydani
Tsukuba, Japan
n6:nazevZdroje
10th International Symposium on Staphylococci and Staphylococcal Infections
n6:obor
n15:EB
n6:pocetDomacichTvurcuVysledku
5
n6:pocetTvurcuVysledku
6
n6:projekt
n17:GA301%2F02%2F1505
n6:rokUplatneniVysledku
n9:2002
n6:tvurceVysledku
Kvardová, Veronika Doškař, Jiří Růžičková, Vladislava Pantůček, Roman Oráčová, Eva Petráš, Petr
n6:typAkce
n11:WRD
n6:zahajeniAkce
2002-01-01+01:00
n6:zamer
n19:MSM%20143100008
s:numberOfPages
1
n13:hasPublisher
Juntendo University
n8:organizacniJednotka
14310