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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F02%3A00006390%21RIV08-GA0-14310___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
Iron is a component of a number of biological systems, e.g. electron transport chains, cofactors of enzymes, regulatory proteins etc. It occurs in ferric form in environment, while organisms require ferrous iron. Enzymes catalysing the reduction of ferric complexes are widely spread within bacterial kingdom. Some of these enzymes are associated with cytoplasmic membrane, some are present in soluble form in cytoplasm or periplasm, some are excreted into extracellular medium. In our work, cellular fractions of Paracoccus denitrificans were tested for their activity towards reduction of various ferric complexes with NADH as an electron donor. We found the most of the ferric reductase activity in cytosolic fraction. Fe(III)-nitrilotriacetate was chosen as the best artificial substrate for activity measurements. Two proteins responsible for the activity was identified. Both enzymes were purified to homogeneity by combination of FPLC ion-exchange chromatography and chromatofocusing, and characterised with r Iron is a component of a number of biological systems, e.g. electron transport chains, cofactors of enzymes, regulatory proteins etc. It occurs in ferric form in environment, while organisms require ferrous iron. Enzymes catalysing the reduction of ferric complexes are widely spread within bacterial kingdom. Some of these enzymes are associated with cytoplasmic membrane, some are present in soluble form in cytoplasm or periplasm, some are excreted into extracellular medium. In our work, cellular fractions of Paracoccus denitrificans were tested for their activity towards reduction of various ferric complexes with NADH as an electron donor. We found the most of the ferric reductase activity in cytosolic fraction. Fe(III)-nitrilotriacetate was chosen as the best artificial substrate for activity measurements. Two proteins responsible for the activity was identified. Both enzymes were purified to homogeneity by combination of FPLC ion-exchange chromatography and chromatofocusing, and characterised with r Iron is a component of a number of biological systems, e.g. electron transport chains, cofactors of enzymes, regulatory proteins etc. It occurs in ferric form in environment, while organisms require ferrous iron. Enzymes catalysing the reduction of ferric complexes are widely spread within bacterial kingdom. Some of these enzymes are associated with cytoplasmic membrane, some are present in soluble form in cytoplasm or periplasm, some are excreted into extracellular medium. In our work, cellular fractions of Paracoccus denitrificans were tested for their activity towards reduction of various ferric complexes with NADH as an electron donor. We found the most of the ferric reductase activity in cytosolic fraction. Fe(III)-nitrilotriacetate was chosen as the best artificial substrate for activity measurements. Two proteins responsible for the activity was identified. Both enzymes were purified to homogeneity by combination of FPLC ion-exchange chromatography and chromatofocusing, and characterised with r
dcterms:title
Purification and characterization of ferric reductases from Paracoccus denitrificans Purification and characterization of ferric reductases from Paracoccus denitrificans Purification and characterization of ferric reductases from Paracoccus denitrificans
skos:prefLabel
Purification and characterization of ferric reductases from Paracoccus denitrificans Purification and characterization of ferric reductases from Paracoccus denitrificans Purification and characterization of ferric reductases from Paracoccus denitrificans
skos:notation
RIV/00216224:14310/02:00006390!RIV08-GA0-14310___
n3:strany
150
n3:aktivita
n14:P
n3:aktivity
P(GA203/01/1589)
n3:dodaniDat
n4:2008
n3:domaciTvurceVysledku
n13:7216513 n13:8171335 n13:3087719
n3:druhVysledku
n9:D
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
661214
n3:idVysledku
RIV/00216224:14310/02:00006390
n3:jazykVysledku
n7:eng
n3:klicovaSlova
ferric reductase; Paracoccus denitrificans; purification
n3:klicoveSlovo
n15:purification n15:Paracoccus%20denitrificans n15:ferric%20reductase
n3:kontrolniKodProRIV
[5FE8A5773F80]
n3:mistoKonaniAkce
10.-13.9.2002 Stará Lesná, Slovenská republika
n3:mistoVydani
Bratislava
n3:nazevZdroje
XVIII. BIOCHEMICKÝ ZJAZD
n3:obor
n20:CE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:projekt
n12:GA203%2F01%2F1589
n3:rokUplatneniVysledku
n4:2002
n3:tvurceVysledku
Mazoch, Jiří Kučera, Igor Tesařík, Radek Turánek, Jaroslav
n3:typAkce
n6:EUR
n3:zahajeniAkce
2002-01-01+01:00
s:numberOfPages
1
n11:hasPublisher
Slovenská spoločnosť pre biochémiu a molekulárnu biológiu pri SAV
n19:organizacniJednotka
14310