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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F02%3A00005658%21RIV09-GA0-14310___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
This report describes the application of electrophoretically mediated microanalysis (EMMA) for the study of kinetic parameters of bi-substrate enzymatic reaction of rhodanese. The Michaelis constants (Km) for both substrates and the effect of temperature on rhodanese reaction were evaluated by means of the combination of the EMMA methodology with a partial filling technique. In this set-up the part of the capillary is filled with the buffer best for the enzymatic reaction whereas the rest of the capillary with the background electrolyte optimal for separation of substrates and products. The enzymatic reaction was performed in 25 mM HEPES buffer (pH 8.5) while the low pH background electrolyte 100 mM beta-alanine - HCl (pH 3.5) was used for separation of substrates and products that are the inorganic anions. The estimated value of Km for thiosulfate of 1.30 x 10-2 M was consistent with previously published values; the Km for cyanide of 7.6 x 10-3 M was determined for the first time. In addition the typ This report describes the application of electrophoretically mediated microanalysis (EMMA) for the study of kinetic parameters of bi-substrate enzymatic reaction of rhodanese. The Michaelis constants (Km) for both substrates and the effect of temperature on rhodanese reaction were evaluated by means of the combination of the EMMA methodology with a partial filling technique. In this set-up the part of the capillary is filled with the buffer best for the enzymatic reaction whereas the rest of the capillary with the background electrolyte optimal for separation of substrates and products. The enzymatic reaction was performed in 25 mM HEPES buffer (pH 8.5) while the low pH background electrolyte 100 mM beta-alanine - HCl (pH 3.5) was used for separation of substrates and products that are the inorganic anions. The estimated value of Km for thiosulfate of 1.30 x 10-2 M was consistent with previously published values; the Km for cyanide of 7.6 x 10-3 M was determined for the first time. In addition the typ This report describes the application of electrophoretically mediated microanalysis (EMMA) for the study of kinetic parameters of bi-substrate enzymatic reaction of rhodanese. The Michaelis constants (Km) for both substrates and the effect of temperature on rhodanese reaction were evaluated by means of the combination of the EMMA methodology with a partial filling technique. In this set-up the part of the capillary is filled with the buffer best for the enzymatic reaction whereas the rest of the capillary with the background electrolyte optimal for separation of substrates and products. The enzymatic reaction was performed in 25 mM HEPES buffer (pH 8.5) while the low pH background electrolyte 100 mM beta-alanine - HCl (pH 3.5) was used for separation of substrates and products that are the inorganic anions. The estimated value of Km for thiosulfate of 1.30 x 10-2 M was consistent with previously published values; the Km for cyanide of 7.6 x 10-3 M was determined for the first time. In addition the typ
dcterms:title
Determination of the kinetic parameters of rhodanese by electrophoretically mediated microanalysis in a partially filled capillary Determination of the kinetic parameters of rhodanese by electrophoretically mediated microanalysis in a partially filled capillary Determination of the kinetic parameters of rhodanese by electrophoretically mediated microanalysis in a partially filled capillary
skos:prefLabel
Determination of the kinetic parameters of rhodanese by electrophoretically mediated microanalysis in a partially filled capillary Determination of the kinetic parameters of rhodanese by electrophoretically mediated microanalysis in a partially filled capillary Determination of the kinetic parameters of rhodanese by electrophoretically mediated microanalysis in a partially filled capillary
skos:notation
RIV/00216224:14310/02:00005658!RIV09-GA0-14310___
n5:aktivita
n8:P
n5:aktivity
P(GA525/00/0785)
n5:cisloPeriodika
7-8
n5:dodaniDat
n15:2009
n5:domaciTvurceVysledku
n12:5235979 n12:7727658
n5:druhVysledku
n9:J
n5:duvernostUdaju
n11:S
n5:entitaPredkladatele
n7:predkladatel
n5:idSjednocenehoVysledku
642777
n5:idVysledku
RIV/00216224:14310/02:00005658
n5:jazykVysledku
n18:eng
n5:klicovaSlova
rhodanese; EMMA
n5:klicoveSlovo
n6:rhodanese n6:EMMA
n5:kodStatuVydavatele
US - Spojené státy americké
n5:kontrolniKodProRIV
[32BB243D0909]
n5:nazevZdroje
Electrophoresis
n5:obor
n14:CE
n5:pocetDomacichTvurcuVysledku
2
n5:pocetTvurcuVysledku
2
n5:projekt
n13:GA525%2F00%2F0785
n5:rokUplatneniVysledku
n15:2002
n5:svazekPeriodika
23
n5:tvurceVysledku
Glatz, Zdeněk Vytisková, Soňa
s:issn
0173-0835
s:numberOfPages
7
n10:organizacniJednotka
14310