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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F01%3A00007524%21RIV08-AV0-14310___
rdf:type
n12:Vysledek skos:Concept
dcterms:description
The dependence of the impedance of the electrode double layer of mercury electrode on frequency around the potentials of the tensammetric peaks of single-stranded and double-helical polynucleotides and DNA was studied. From the frequency dependence of the impedance of the electrode double layer represented in a complex impedance plot (the imaginary component Z'' is plotted against the real component Z', Cole-Cole or Nyquist plot) the electric equivalent circuit of the electrode covered with adsorbed DNA layer was determined. It was concluded the desorption of denatured ssDNA is accompanied by higher dielectric losses than the desorption of native dsDNA. This can be explained by the higher flexibility of ssDNA compared to the dsDNA. The capacitance peak of single stranded poly A observed at pH 8 around -1.3 V splits at low frequencies in two peaks. The dependence of the impedance of the electrode double layer of mercury electrode on frequency around the potentials of the tensammetric peaks of single-stranded and double-helical polynucleotides and DNA was studied. From the frequency dependence of the impedance of the electrode double layer represented in a complex impedance plot (the imaginary component Z'' is plotted against the real component Z', Cole-Cole or Nyquist plot) the electric equivalent circuit of the electrode covered with adsorbed DNA layer was determined. It was concluded the desorption of denatured ssDNA is accompanied by higher dielectric losses than the desorption of native dsDNA. This can be explained by the higher flexibility of ssDNA compared to the dsDNA. The capacitance peak of single stranded poly A observed at pH 8 around -1.3 V splits at low frequencies in two peaks. The dependence of the impedance of the electrode double layer of mercury electrode on frequency around the potentials of the tensammetric peaks of single-stranded and double-helical polynucleotides and DNA was studied. From the frequency dependence of the impedance of the electrode double layer represented in a complex impedance plot (the imaginary component Z'' is plotted against the real component Z', Cole-Cole or Nyquist plot) the electric equivalent circuit of the electrode covered with adsorbed DNA layer was determined. It was concluded the desorption of denatured ssDNA is accompanied by higher dielectric losses than the desorption of native dsDNA. This can be explained by the higher flexibility of ssDNA compared to the dsDNA. The capacitance peak of single stranded poly A observed at pH 8 around -1.3 V splits at low frequencies in two peaks.
dcterms:title
Electrochemical impedance spectroscopy of single-stranded and double-helical polynucleotides Electrochemical impedance spectroscopy of single-stranded and double-helical polynucleotides Electrochemical impedance spectroscopy of single-stranded and double-helical polynucleotides
skos:prefLabel
Electrochemical impedance spectroscopy of single-stranded and double-helical polynucleotides Electrochemical impedance spectroscopy of single-stranded and double-helical polynucleotides Electrochemical impedance spectroscopy of single-stranded and double-helical polynucleotides
skos:notation
RIV/00216224:14310/01:00007524!RIV08-AV0-14310___
n3:strany
29
n3:aktivita
n10:P
n3:aktivity
P(GV204/97/K084), P(IAA4004002)
n3:dodaniDat
n11:2008
n3:domaciTvurceVysledku
n7:8856486 n7:4637844 n7:7668473 n7:5374650 n7:1471163 n7:7746547
n3:druhVysledku
n16:D
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
679096
n3:idVysledku
RIV/00216224:14310/01:00007524
n3:jazykVysledku
n17:eng
n3:klicovaSlova
electrochemical impedance spectroscopy; EIS; DNA adsorption; polyadenylic acid adsorption
n3:klicoveSlovo
n5:electrochemical%20impedance%20spectroscopy n5:polyadenylic%20acid%20adsorption n5:EIS n5:DNA%20adsorption
n3:kontrolniKodProRIV
[085E4DB26213]
n3:mistoKonaniAkce
30.5.-1.6.2001, Mozolov u Tábora
n3:mistoVydani
Mozolov u Tábora
n3:nazevZdroje
Book of Abstracts, XXIV.dny lékařské biofyziky
n3:obor
n8:BO
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n4:IAA4004002 n4:GV204%2F97%2FK084
n3:rokUplatneniVysledku
n11:2001
n3:tvurceVysledku
Hasoň, Stanislav Vetterl, Vladimír Jelen, František Dražan, Viktor Strašák, Luděk Dvořák, Jakub
n3:typAkce
n20:EUR
n3:zahajeniAkce
2001-01-01+01:00
s:numberOfPages
1
n15:hasPublisher
XXIV.dny lékařské biofyziky
n13:organizacniJednotka
14310