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Statements

Subject Item
n2:RIV%2F00216224%3A14310%2F01%3A00004808%21RIV08-GA0-14310___
rdf:type
n13:Vysledek skos:Concept
dcterms:description
The purpose of the present study was to investigate the application of various sample preparation methods (cell washing before lysis, purification of DNA using phenol extraction method, immunomagnetic separation-IMS) for the final PCR identification of Salmonella cells. The presence of PCR inhibitors in processed food products (milk powder and dried eggs) can be the cause of false-negative results in PCR without IMS of target cells. It was also demonstrated that IMS-PCR was succesfully used for identification and quick confirmation of untypical Salmonella strains isolated from human stool samples and rabbit meat. However, IMS cannot eliminate intracellular PCR inhibitors present in immunoseparated Salmonella cells. These inhibitors must be taken into consideration in evaluation of PCR procedure. The purpose of the present study was to investigate the application of various sample preparation methods (cell washing before lysis, purification of DNA using phenol extraction method, immunomagnetic separation-IMS) for the final PCR identification of Salmonella cells. The presence of PCR inhibitors in processed food products (milk powder and dried eggs) can be the cause of false-negative results in PCR without IMS of target cells. It was also demonstrated that IMS-PCR was succesfully used for identification and quick confirmation of untypical Salmonella strains isolated from human stool samples and rabbit meat. However, IMS cannot eliminate intracellular PCR inhibitors present in immunoseparated Salmonella cells. These inhibitors must be taken into consideration in evaluation of PCR procedure. The purpose of the present study was to investigate the application of various sample preparation methods (cell washing before lysis, purification of DNA using phenol extraction method, immunomagnetic separation-IMS) for the final PCR identification of Salmonella cells. The presence of PCR inhibitors in processed food products (milk powder and dried eggs) can be the cause of false-negative results in PCR without IMS of target cells. It was also demonstrated that IMS-PCR was succesfully used for identification and quick confirmation of untypical Salmonella strains isolated from human stool samples and rabbit meat. However, IMS cannot eliminate intracellular PCR inhibitors present in immunoseparated Salmonella cells. These inhibitors must be taken into consideration in evaluation of PCR procedure.
dcterms:title
PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation
skos:prefLabel
PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation
skos:notation
RIV/00216224:14310/01:00004808!RIV08-GA0-14310___
n3:strany
379
n3:aktivita
n18:P
n3:aktivity
P(GA203/98/1231), P(IZ3988)
n3:cisloPeriodika
6
n3:dodaniDat
n15:2008
n3:domaciTvurceVysledku
n7:8264945 n7:2412594 n7:5623596 n7:4977459
n3:druhVysledku
n5:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
690831
n3:idVysledku
RIV/00216224:14310/01:00004808
n3:jazykVysledku
n4:eng
n3:klicovaSlova
Salmonella; PCR; IMS; inhibitors; food and stool samples
n3:klicoveSlovo
n8:PCR n8:IMS n8:Salmonella n8:food%20and%20stool%20samples n8:inhibitors
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[A3CD7CABE715]
n3:nazevZdroje
Bioseparation
n3:obor
n17:EE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
5
n3:projekt
n11:IZ3988 n11:GA203%2F98%2F1231
n3:rokUplatneniVysledku
n15:2001
n3:svazekPeriodika
9
n3:tvurceVysledku
Španová, Alena Štreitová, Denisa Rittich, Bohuslav Čechová, Leona Karpíšková, Renata
s:issn
0923-179X
s:numberOfPages
6
n10:organizacniJednotka
14310