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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F99%3A00001800%21RIV09-MSM-14110___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
The presence of laminin-1, collagen-IV, a6 and b1 integrin chains was detected by indirect immunohistochemistry using biotin/streptavidin/HRP or gold-conjugated secondary antibody at the light and electron microscope level, respectively. Cryo-treated segment of the peripheral stump without living Schwann cells (S-100-) did not display immunoreactivity for laminin-1 and integrin's chains, while the migrating Schwann cells in the marginal regions were immunostained for the antigens. Isolated acellular nerve segments protected from migration of Schwann cells (S-100-) exhibited laminin-l-, b1-, and a6- integrin chains immunoreactivities. Position of the basal lamina was verified by collagen-IV+ immunoreactivity. Results indicate that presence of the laminin in the peripheral nerve is related with living Schwann cells. The presence of laminin-1, collagen-IV, a6 and b1 integrin chains was detected by indirect immunohistochemistry using biotin/streptavidin/HRP or gold-conjugated secondary antibody at the light and electron microscope level, respectively. Cryo-treated segment of the peripheral stump without living Schwann cells (S-100-) did not display immunoreactivity for laminin-1 and integrin's chains, while the migrating Schwann cells in the marginal regions were immunostained for the antigens. Isolated acellular nerve segments protected from migration of Schwann cells (S-100-) exhibited laminin-l-, b1-, and a6- integrin chains immunoreactivities. Position of the basal lamina was verified by collagen-IV+ immunoreactivity. Results indicate that presence of the laminin in the peripheral nerve is related with living Schwann cells. Imunohistochemická detekce lamininu-1, collagen-IV, a6 a b1 řetězce integrinů byla studována v acelulárním segmentu nervu
dcterms:title
Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors Imunohistochemická analýza lamininu v acelulárním nervovém štěpu Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors
skos:prefLabel
Imunohistochemická analýza lamininu v acelulárním nervovém štěpu Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors
skos:notation
RIV/00216224:14110/99:00001800!RIV09-MSM-14110___
n3:aktivita
n6:Z
n3:aktivity
Z(MSM 141100001)
n3:cisloPeriodika
1
n3:dodaniDat
n7:2009
n3:domaciTvurceVysledku
n9:6754937 n9:5568692 n9:1604074 n9:7731361
n3:druhVysledku
n10:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
742382
n3:idVysledku
RIV/00216224:14110/99:00001800
n3:jazykVysledku
n15:eng
n3:klicovaSlova
Acellular nerve graft; Integrins; Laminin-1; Schwann cell migration
n3:klicoveSlovo
n12:Laminin-1 n12:Schwann%20cell%20migration n12:Acellular%20nerve%20graft n12:Integrins
n3:kodStatuVydavatele
SK - Slovenská republika
n3:kontrolniKodProRIV
[F4A237F26470]
n3:nazevZdroje
General Physiology and Biophysics
n3:obor
n13:FH
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n7:1999
n3:svazekPeriodika
18
n3:tvurceVysledku
Hradilová Svíženská, Ivana Haninec, Pavel Houšťava, Ladislav Jančálek, Radim Dubový, Petr Klusáková, Ilona
n3:zamer
n11:MSM%20141100001
s:issn
0231-5882
s:numberOfPages
3
n18:organizacniJednotka
14110