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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F13%3A00065613%21RIV14-MZ0-14110___
rdf:type
skos:Concept n13:Vysledek
dcterms:description
This study examined the sequences of the two rRNA (rrn) operons of pathogenic non-cultivable treponemes, comprising 11 strains of T. pallidum ssp. pallidum (TPA), five strains of T. pallidum ssp. pertenue (TPE), two strains of T. pallidum ssp. endemicum (TEN), a simian Fribourg-Blanc strain and a rabbit T. paraluiscuniculi (TPc) strain. PCR was used to determine the type of 16S- 23S ribosomal intergenic spacers in the rrn operons from 30 clinical samples belonging to five different genotypes. When compared with the TPA strains, TPc Cuniculi A strain had a 17 bp deletion, and the TPE, TEN and Fribourg-Blanc isolates had a deletion of 33 bp. Other than these deletions, only 17 heterogeneous sites were found within the entire region (excluding the 16S- 23S intergenic spacer region encoding tRNA-Ile or tRNA-Ala). This study examined the sequences of the two rRNA (rrn) operons of pathogenic non-cultivable treponemes, comprising 11 strains of T. pallidum ssp. pallidum (TPA), five strains of T. pallidum ssp. pertenue (TPE), two strains of T. pallidum ssp. endemicum (TEN), a simian Fribourg-Blanc strain and a rabbit T. paraluiscuniculi (TPc) strain. PCR was used to determine the type of 16S- 23S ribosomal intergenic spacers in the rrn operons from 30 clinical samples belonging to five different genotypes. When compared with the TPA strains, TPc Cuniculi A strain had a 17 bp deletion, and the TPE, TEN and Fribourg-Blanc isolates had a deletion of 33 bp. Other than these deletions, only 17 heterogeneous sites were found within the entire region (excluding the 16S- 23S intergenic spacer region encoding tRNA-Ile or tRNA-Ala).
dcterms:title
Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains
skos:prefLabel
Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains
skos:notation
RIV/00216224:14110/13:00065613!RIV14-MZ0-14110___
n13:predkladatel
n14:orjk%3A14110
n3:aktivita
n12:P
n3:aktivity
P(GAP302/12/0574), P(NT11159)
n3:cisloPeriodika
2
n3:dodaniDat
n8:2014
n3:domaciTvurceVysledku
n9:2170620 n9:8932395 n9:4942558 n9:1959468 n9:5235383 n9:9995307
n3:druhVysledku
n16:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
108416
n3:idVysledku
RIV/00216224:14110/13:00065613
n3:jazykVysledku
n4:eng
n3:klicovaSlova
article; bacterial strain; bacterium isolate; controlled study; gene deletion; genotype; nonhuman; operon; polymerase chain reaction; priority journal; RNA sequence; Treponema; Treponema pallidum; Treponema paraluiscuniculi
n3:klicoveSlovo
n7:bacterium%20isolate n7:Treponema n7:gene%20deletion n7:bacterial%20strain n7:article n7:nonhuman n7:genotype n7:operon n7:polymerase%20chain%20reaction n7:priority%20journal n7:RNA%20sequence n7:Treponema%20pallidum n7:Treponema%20paraluiscuniculi n7:controlled%20study
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[96056229E4F1]
n3:nazevZdroje
Journal of Medical Microbiology
n3:obor
n15:EE
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
7
n3:projekt
n19:GAP302%2F12%2F0574 n19:NT11159
n3:rokUplatneniVysledku
n8:2013
n3:svazekPeriodika
62
n3:tvurceVysledku
Weinstock, George M. Mikalová, Lenka Strouhal, Michal Pospíšilová, Petra Zobaníková, Marie Čejková, Darina Šmajs, David
n3:wos
000315554000004
s:issn
0022-2615
s:numberOfPages
12
n20:doi
10.1099/jmm.0.050658-0
n10:organizacniJednotka
14110