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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F13%3A00065612%21RIV14-MZ0-14110___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Background: Unclassified simian strain Treponema Fribourg-Blanc was isolated in 1966 from baboons (Papio cynocephalus)in West Africa. This strain was morphologically indistinguishable from T. pallidum ssp. pallidum or ssp. pertenue strains, and it was shown to cause human infections. Methodology/Principal Findings: To precisely define genetic differences between Treponema Fribourg-Blanc (unclassified simian isolate, FB) and T. pallidum ssp. pertenue strains (TPE), a high quality sequence of the whole Fribourg-Blanc genome was determined with 454-pyrosequencing and Illumina sequencing platforms. Combined average coverage of both methods was greater than 5006. Restriction target sites (n = 1,773), identified in silico, of selected restriction enzymes within the Fribourg-Blanc genome were verified experimentally and no discrepancies were found. When compared to the other three sequenced TPE genomes (Samoa D, CDC-2, Gauthier), no major genome rearrangements were found. Background: Unclassified simian strain Treponema Fribourg-Blanc was isolated in 1966 from baboons (Papio cynocephalus)in West Africa. This strain was morphologically indistinguishable from T. pallidum ssp. pallidum or ssp. pertenue strains, and it was shown to cause human infections. Methodology/Principal Findings: To precisely define genetic differences between Treponema Fribourg-Blanc (unclassified simian isolate, FB) and T. pallidum ssp. pertenue strains (TPE), a high quality sequence of the whole Fribourg-Blanc genome was determined with 454-pyrosequencing and Illumina sequencing platforms. Combined average coverage of both methods was greater than 5006. Restriction target sites (n = 1,773), identified in silico, of selected restriction enzymes within the Fribourg-Blanc genome were verified experimentally and no discrepancies were found. When compared to the other three sequenced TPE genomes (Samoa D, CDC-2, Gauthier), no major genome rearrangements were found.
dcterms:title
Whole Genome Sequence of the Treponema Fribourg-Blanc: Unspecified Simian Isolate Is Highly Similar to the Yaws Subspecies Whole Genome Sequence of the Treponema Fribourg-Blanc: Unspecified Simian Isolate Is Highly Similar to the Yaws Subspecies
skos:prefLabel
Whole Genome Sequence of the Treponema Fribourg-Blanc: Unspecified Simian Isolate Is Highly Similar to the Yaws Subspecies Whole Genome Sequence of the Treponema Fribourg-Blanc: Unspecified Simian Isolate Is Highly Similar to the Yaws Subspecies
skos:notation
RIV/00216224:14110/13:00065612!RIV14-MZ0-14110___
n18:predkladatel
n19:orjk%3A14110
n3:aktivita
n14:P
n3:aktivity
P(GAP302/12/0574), P(NT11159)
n3:cisloPeriodika
4
n3:dodaniDat
n8:2014
n3:domaciTvurceVysledku
n5:4942558 n5:8932395 n5:1959468 n5:1343408 n5:5235383 n5:9995307 n5:2170620
n3:druhVysledku
n15:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
117341
n3:idVysledku
RIV/00216224:14110/13:00065612
n3:jazykVysledku
n4:eng
n3:klicovaSlova
PALLIDUM SSP PALLIDUM; PATHOGENIC TREPONEMES; GENE-EXPRESSION; SYPHILIS; STRAINS; CONSERVATION; PERTENUE; LIPOPROTEIN; INFECTION; DISEASE
n3:klicoveSlovo
n6:DISEASE n6:LIPOPROTEIN n6:PALLIDUM%20SSP%20PALLIDUM n6:PATHOGENIC%20TREPONEMES n6:PERTENUE n6:CONSERVATION n6:SYPHILIS n6:STRAINS n6:GENE-EXPRESSION n6:INFECTION
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[AEE2854C426D]
n3:nazevZdroje
PLOS Neglected Tropical Diseases
n3:obor
n13:EE
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
11
n3:projekt
n10:GAP302%2F12%2F0574 n10:NT11159
n3:rokUplatneniVysledku
n8:2013
n3:svazekPeriodika
7
n3:tvurceVysledku
Weinstock, George M. Čejková, Darina Pospíšilová, Petra Mikalová, Lenka Chen, Lei Fulton, Lucinda L. Ambrožová, Lenka Šmajs, David Zobaníková, Marie Sodergren, Erica Strouhal, Michal
n3:wos
000318153100026
s:issn
1935-2735
s:numberOfPages
8
n11:doi
10.1371/journal.pntd.0002172
n20:organizacniJednotka
14110