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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F11%3A00053108%21RIV12-MSM-14110___
rdf:type
skos:Concept n9:Vysledek
dcterms:description
The aim of this study was to evolve an effective procedure for the sperm lysis, obtention of intracellular content and subsequent assesment of Hc concentration and, in consequence, determine the correlations between blood plasma and seminal fluid Hc concentration, spermiogram parameters and DNA injury. Firstly, we used the method of repeated freezing and melting according to Ebisch et al., 2006 for the sperm lysis. Because the microscopic control of the samples didn’t show any markable sperm injury, we projected and compared other methods of the cell lysis – ultrasound, melting in ultrasound and freezing 10 times, methanol, distilled water, Triton X-100 solution, and lysis solution + 1% Triton X-100 solution. We used the method of high-performance liquid chromatography with fluorescence detection (HPLCFD) for the determination of Hc concentration and comet analysis for the assesment of DNA injury. The aim of this study was to evolve an effective procedure for the sperm lysis, obtention of intracellular content and subsequent assesment of Hc concentration and, in consequence, determine the correlations between blood plasma and seminal fluid Hc concentration, spermiogram parameters and DNA injury. Firstly, we used the method of repeated freezing and melting according to Ebisch et al., 2006 for the sperm lysis. Because the microscopic control of the samples didn’t show any markable sperm injury, we projected and compared other methods of the cell lysis – ultrasound, melting in ultrasound and freezing 10 times, methanol, distilled water, Triton X-100 solution, and lysis solution + 1% Triton X-100 solution. We used the method of high-performance liquid chromatography with fluorescence detection (HPLCFD) for the determination of Hc concentration and comet analysis for the assesment of DNA injury.
dcterms:title
Assesment of intracellular homocysteine concentration in sperms Assesment of intracellular homocysteine concentration in sperms
skos:prefLabel
Assesment of intracellular homocysteine concentration in sperms Assesment of intracellular homocysteine concentration in sperms
skos:notation
RIV/00216224:14110/11:00053108!RIV12-MSM-14110___
n9:predkladatel
n10:orjk%3A14110
n3:aktivita
n8:V
n3:aktivity
V
n3:dodaniDat
n6:2012
n3:domaciTvurceVysledku
n4:7982186 n4:4996291 n4:9456384 n4:3657183 n4:1241184 n4:4222229
n3:druhVysledku
n17:O
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
187219
n3:idVysledku
RIV/00216224:14110/11:00053108
n3:jazykVysledku
n16:eng
n3:klicovaSlova
intracellular homocysteine sperms
n3:klicoveSlovo
n14:intracellular%20homocysteine%20sperms
n3:kontrolniKodProRIV
[5D08FEBBEC66]
n3:obor
n13:FK
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
8
n3:rokUplatneniVysledku
n6:2011
n3:tvurceVysledku
Matejovičová, Milena Vodová, M. Ventruba, Pavel Králíková, Michaela Crha, Igor Lousová, E. Melounová, Jitka Žáková, Jana
n15:organizacniJednotka
14110