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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F11%3A00050081%21RIV12-GA0-14110___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
Temperature-sensitive actin mutant of Saccharomyces cerevisiae act1-1 was studied at a permissive temperature of 23 C by light, fluorescent and electron microscopy to elucidate the roles of actin cytoskeleton in the cycling eukaryotic cells. Mutant cells that grew slowly at the permissive temperature showed aberrations in the cytoskeleton and cell cycle. Mutant cells contained aberrant faint actin cables, that failed in directing of mitochondria, vacuoles and secretory vesicles to the bud and the stray vesicles delivered their content to the mother wall instead of the bud. Bud growth was delayed. Spindle pole bodies and cytoplasmic microtubules did not direct to the bud, and nucleus failed to migrate to the bud. Repeated nuclear divisions produced multinucleated cells, indicating continued cycling of actin mutant cells that failed in the morphogenetic checkpoint, the spindle position checkpoint and cytokinesis. Temperature-sensitive actin mutant of Saccharomyces cerevisiae act1-1 was studied at a permissive temperature of 23 C by light, fluorescent and electron microscopy to elucidate the roles of actin cytoskeleton in the cycling eukaryotic cells. Mutant cells that grew slowly at the permissive temperature showed aberrations in the cytoskeleton and cell cycle. Mutant cells contained aberrant faint actin cables, that failed in directing of mitochondria, vacuoles and secretory vesicles to the bud and the stray vesicles delivered their content to the mother wall instead of the bud. Bud growth was delayed. Spindle pole bodies and cytoplasmic microtubules did not direct to the bud, and nucleus failed to migrate to the bud. Repeated nuclear divisions produced multinucleated cells, indicating continued cycling of actin mutant cells that failed in the morphogenetic checkpoint, the spindle position checkpoint and cytokinesis.
dcterms:title
Ultrastructural disorder of actin mutant suggests uncoupling of actin-dependent pathway from microtubule-dependent pathway in budding yeast Ultrastructural disorder of actin mutant suggests uncoupling of actin-dependent pathway from microtubule-dependent pathway in budding yeast
skos:prefLabel
Ultrastructural disorder of actin mutant suggests uncoupling of actin-dependent pathway from microtubule-dependent pathway in budding yeast Ultrastructural disorder of actin mutant suggests uncoupling of actin-dependent pathway from microtubule-dependent pathway in budding yeast
skos:notation
RIV/00216224:14110/11:00050081!RIV12-GA0-14110___
n10:predkladatel
n16:orjk%3A14110
n3:aktivita
n14:P
n3:aktivity
P(GA310/06/0605)
n3:cisloPeriodika
6
n3:dodaniDat
n5:2012
n3:domaciTvurceVysledku
n13:3299562
n3:druhVysledku
n15:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n20:predkladatel
n3:idSjednocenehoVysledku
236689
n3:idVysledku
RIV/00216224:14110/11:00050081
n3:jazykVysledku
n12:eng
n3:klicovaSlova
actin; nuclear position; organelle inheritance; microtubules
n3:klicoveSlovo
n4:organelle%20inheritance n4:nuclear%20position n4:actin n4:microtubules
n3:kodStatuVydavatele
JP - Japonsko
n3:kontrolniKodProRIV
[76D00DDF6542]
n3:nazevZdroje
Journal of Electron Microscopy
n3:obor
n18:EE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
2
n3:projekt
n8:GA310%2F06%2F0605
n3:rokUplatneniVysledku
n5:2011
n3:svazekPeriodika
60
n3:tvurceVysledku
Yamaguchi, Masashi Kopecká, Marie
n3:wos
000297867800005
s:issn
0022-0744
s:numberOfPages
13
n6:doi
10.1093/jmicro/dfr073
n19:organizacniJednotka
14110