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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F10%3A00051723%21RIV12-MSM-14110___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
The study was carried out to evaluate the effect of several substrates on oxidative stress induced apoptosis and in K-562 cells. Methods. Glucose at 5, 11 and 30 mM concentrations was tested, as well as 5 mM glutamine and 5 mM fructose. The cells were exposed to tert-butylhydroperoxide (tBH) and apoptotic cells were evaluated by flow cytometry with FITC-Annexin V and propidium iodide. The effect of glucose concentration on DNA damage was evaluated using hydrogen peroxide and electrophoretic “DNA comets” assay at 5 mM and 30 mM glucose concentrations. Results. The exposure of cells to tBH resulted in increased number of apoptotic cells, and this effect was prevented by administration of an antioxidant – N-Acetyl cysteine. Rising concentrations of glucose added to the toxic effect of tBH; we also observed some toxic effect of fructose and no effect of glutamine. We found higher susceptibility to hydrogen peroxide induced DNA damage with 30 mM glucose concentration. Conclusion. The study was carried out to evaluate the effect of several substrates on oxidative stress induced apoptosis and in K-562 cells. Methods. Glucose at 5, 11 and 30 mM concentrations was tested, as well as 5 mM glutamine and 5 mM fructose. The cells were exposed to tert-butylhydroperoxide (tBH) and apoptotic cells were evaluated by flow cytometry with FITC-Annexin V and propidium iodide. The effect of glucose concentration on DNA damage was evaluated using hydrogen peroxide and electrophoretic “DNA comets” assay at 5 mM and 30 mM glucose concentrations. Results. The exposure of cells to tBH resulted in increased number of apoptotic cells, and this effect was prevented by administration of an antioxidant – N-Acetyl cysteine. Rising concentrations of glucose added to the toxic effect of tBH; we also observed some toxic effect of fructose and no effect of glutamine. We found higher susceptibility to hydrogen peroxide induced DNA damage with 30 mM glucose concentration. Conclusion.
dcterms:title
HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS
skos:prefLabel
HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS
skos:notation
RIV/00216224:14110/10:00051723!RIV12-MSM-14110___
n3:aktivita
n17:I
n3:aktivity
I
n3:cisloPeriodika
4
n3:dodaniDat
n7:2012
n3:domaciTvurceVysledku
n12:1852698 n12:7273320
n3:druhVysledku
n15:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
261506
n3:idVysledku
RIV/00216224:14110/10:00051723
n3:jazykVysledku
n13:eng
n3:klicovaSlova
K-562 cells; Oxidative stress; Apoptosis; Energy substrates; Hyperglycemia; DNA damage
n3:klicoveSlovo
n4:Hyperglycemia n4:Energy%20substrates n4:K-562%20cells n4:Apoptosis n4:Oxidative%20stress n4:DNA%20damage
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[92130CA74A75]
n3:nazevZdroje
Biomedical Papers
n3:obor
n11:FP
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n7:2010
n3:svazekPeriodika
154
n3:tvurceVysledku
Hruda, Jan Leverve, Xavier Šrámek, Vladimír
n3:wos
000285643800007
s:issn
1213-8118
s:numberOfPages
6
n10:organizacniJednotka
14110