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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F07%3A00048140%21RIV11-GA0-14110___
rdf:type
n5:Vysledek skos:Concept
dcterms:description
Voltage-sensitive dyes (VSD's) undergo changes in their electronic structure, and consequently their fluorescence spectra, in response to changes in the surrounding electric field and are therefore used for recording of monophasic action potentials (MAPs) by optical method in animal hearts. VSD di-4-ANEPPS is employed in our laboratory to record MAPs in isolated rabbit hearts perfused according to Langendorff with Krebs-Henseleit solution (K-H, 37C, 1, 25mM Ca2+). After stabilization (30min), the tissue is stained with di-4-ANEPPS (2mM) diluted in K-H (20-25min) and then washed-out with dye-free K-H (20-25min). Then measurement of MAPs is performed. So called photodynamic damage or phototoxic effects of VSDs have been reported under intense illumination, with alteration of electrical activity of the spontaneously beating heart. In order to determine whether this side effect is present in our experimental model, we followed electrogram, mean coronary flow and formation of hydroxyl radicals. Voltage-sensitive dyes (VSD's) undergo changes in their electronic structure, and consequently their fluorescence spectra, in response to changes in the surrounding electric field and are therefore used for recording of monophasic action potentials (MAPs) by optical method in animal hearts. VSD di-4-ANEPPS is employed in our laboratory to record MAPs in isolated rabbit hearts perfused according to Langendorff with Krebs-Henseleit solution (K-H, 37C, 1, 25mM Ca2+). After stabilization (30min), the tissue is stained with di-4-ANEPPS (2mM) diluted in K-H (20-25min) and then washed-out with dye-free K-H (20-25min). Then measurement of MAPs is performed. So called photodynamic damage or phototoxic effects of VSDs have been reported under intense illumination, with alteration of electrical activity of the spontaneously beating heart. In order to determine whether this side effect is present in our experimental model, we followed electrogram, mean coronary flow and formation of hydroxyl radicals.
dcterms:title
Tissue Response during Staining and Illumination of Voltage-Sensitive Dye in Rabbit Myocardium Tissue Response during Staining and Illumination of Voltage-Sensitive Dye in Rabbit Myocardium
skos:prefLabel
Tissue Response during Staining and Illumination of Voltage-Sensitive Dye in Rabbit Myocardium Tissue Response during Staining and Illumination of Voltage-Sensitive Dye in Rabbit Myocardium
skos:notation
RIV/00216224:14110/07:00048140!RIV11-GA0-14110___
n3:aktivita
n12:P
n3:aktivity
P(GA102/07/1473)
n3:dodaniDat
n15:2011
n3:domaciTvurceVysledku
n13:5074169 n13:1469517
n3:druhVysledku
n4:D
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
455237
n3:idVysledku
RIV/00216224:14110/07:00048140
n3:jazykVysledku
n6:eng
n3:klicovaSlova
voltage-sensitive dye; rabbit myocardium; staining; illumination
n3:klicoveSlovo
n11:staining n11:voltage-sensitive%20dye n11:illumination n11:rabbit%20myocardium
n3:kontrolniKodProRIV
[8D936E1AEDE3]
n3:mistoKonaniAkce
Durham, USA
n3:mistoVydani
Durham, USA
n3:nazevZdroje
Computers in Cardiology
n3:obor
n19:ED
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n9:GA102%2F07%2F1473
n3:rokUplatneniVysledku
n15:2007
n3:tvurceVysledku
Fialová, Kateřina Nováková, Marie Provazník, Ivo Bardoňová, Jana
n3:typAkce
n20:WRD
n3:zahajeniAkce
2007-09-30+02:00
s:issn
0276-6574
s:numberOfPages
4
n17:hasPublisher
IEEE
n16:organizacniJednotka
14110