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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F07%3A00020661%21RIV10-GA0-14110___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
Background: Quaternary benzo[c]phenanthridine alkaloids (QBAs) are naturally occurring compounds isolated from plants of Fumariaceae, Papaveraceae, Ranunculaceae and Rutaceae families. In addition to wide biological activities, they are attractive for their fluorescence. We observed interesting fluorescent characteristics of QBAs - macarpine (MA), sanguirubine (SR), chelirubine (CHR), sanguilutine (SL), chelilutine (CHL), sanguinarine (SA) and chelerythrine (CHE) upon interacting with living cells. Methods: Water stock solutions of the alkaloids (10-100 mg/ml) were added to intact cells and upon brief incubation the alkaloids stain the cells. Human cell lines HL60 (human promyelocytic leukemia), HeLa (human cervix adenocarcinoma) and LEP (human lung fibroblasts) and piglet blood were used in experiments. Blood cells were stained with MA in a combination with FITC-conjugated anti CD45 surface marker antibody. Cells were analysed by fluorescence microscopy and by flow cytometry. Background: Quaternary benzo[c]phenanthridine alkaloids (QBAs) are naturally occurring compounds isolated from plants of Fumariaceae, Papaveraceae, Ranunculaceae and Rutaceae families. In addition to wide biological activities, they are attractive for their fluorescence. We observed interesting fluorescent characteristics of QBAs - macarpine (MA), sanguirubine (SR), chelirubine (CHR), sanguilutine (SL), chelilutine (CHL), sanguinarine (SA) and chelerythrine (CHE) upon interacting with living cells. Methods: Water stock solutions of the alkaloids (10-100 mg/ml) were added to intact cells and upon brief incubation the alkaloids stain the cells. Human cell lines HL60 (human promyelocytic leukemia), HeLa (human cervix adenocarcinoma) and LEP (human lung fibroblasts) and piglet blood were used in experiments. Blood cells were stained with MA in a combination with FITC-conjugated anti CD45 surface marker antibody. Cells were analysed by fluorescence microscopy and by flow cytometry.
dcterms:title
Quaternary Benzo[c]phenanthridine Alkaloids - Novel Cell Permeant and Red Fluorescing DNA Probes Quaternary Benzo[c]phenanthridine Alkaloids - Novel Cell Permeant and Red Fluorescing DNA Probes
skos:prefLabel
Quaternary Benzo[c]phenanthridine Alkaloids - Novel Cell Permeant and Red Fluorescing DNA Probes Quaternary Benzo[c]phenanthridine Alkaloids - Novel Cell Permeant and Red Fluorescing DNA Probes
skos:notation
RIV/00216224:14110/07:00020661!RIV10-GA0-14110___
n3:aktivita
n12:P n12:Z
n3:aktivity
P(GA525/04/0017), P(LC06077), Z(MSM0021622415)
n3:cisloPeriodika
9
n3:dodaniDat
n5:2010
n3:domaciTvurceVysledku
n4:3682455 n4:2115549 n4:9540539
n3:druhVysledku
n15:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
446200
n3:idVysledku
RIV/00216224:14110/07:00020661
n3:jazykVysledku
n19:eng
n3:klicovaSlova
benzophenanthridine alkaloids; cell-permeant DNA dye; chelirubine; flow cytometry; fluorescence microscopy; DNA content; cell cycle; macarpine; multicolor analysis; sanguinarine
n3:klicoveSlovo
n7:sanguinarine n7:multicolor%20analysis n7:DNA%20content n7:flow%20cytometry n7:cell%20cycle n7:chelirubine n7:benzophenanthridine%20alkaloids n7:cell-permeant%20DNA%20dye n7:fluorescence%20microscopy n7:macarpine
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[FC02A962C750]
n3:nazevZdroje
Cytometry Part A
n3:obor
n18:EA
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n11:LC06077 n11:GA525%2F04%2F0017
n3:rokUplatneniVysledku
n5:2007
n3:svazekPeriodika
71A
n3:tvurceVysledku
Táborská, Eva Slaninová, Iva Slanina, Jiří
n3:wos
000249187800011
n3:zamer
n17:MSM0021622415
s:issn
1552-4922
s:numberOfPages
9
n13:organizacniJednotka
14110