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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F06%3A00018909%21RIV10-MZ0-14110___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4+ and CD8+ T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 e06 T cells. Specificity of the T lymphocytes was tested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)–based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitro to numbers useful for clinical applications. Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4+ and CD8+ T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 e06 T cells. Specificity of the T lymphocytes was tested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)–based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitro to numbers useful for clinical applications.
dcterms:title
Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy
skos:prefLabel
Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy
skos:notation
RIV/00216224:14110/06:00018909!RIV10-MZ0-14110___
n3:aktivita
n18:P
n3:aktivity
P(NC7475)
n3:cisloPeriodika
3
n3:dodaniDat
n7:2010
n3:domaciTvurceVysledku
n4:9622748 n4:2973561 n4:4954130 n4:1873172 n4:5551579 n4:6522319 n4:4648137
n3:druhVysledku
n16:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n5:predkladatel
n3:idSjednocenehoVysledku
480511
n3:idVysledku
RIV/00216224:14110/06:00018909
n3:jazykVysledku
n13:eng
n3:klicovaSlova
Multiple myeloma; immunotherapy; interferon-gamma; T cell; CFSE
n3:klicoveSlovo
n11:T%20cell n11:CFSE n11:immunotherapy n11:Multiple%20myeloma n11:interferon-gamma
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[3C494365CB76]
n3:nazevZdroje
Medical Oncology
n3:obor
n6:FD
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
7
n3:projekt
n17:NC7475
n3:rokUplatneniVysledku
n7:2006
n3:svazekPeriodika
23/2006
n3:tvurceVysledku
Žaloudík, Jan Kovářová, Lucie Zahradová, Lenka Očadlíková, Darina Hájek, Roman Michálek, Jaroslav Penka, Miroslav
n3:wos
000242136000009
s:issn
1559-131X
s:numberOfPages
8
n15:organizacniJednotka
14110