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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F06%3A00016450%21RIV10-MSM-14110___
rdf:type
n12:Vysledek skos:Concept
dcterms:description
The nonionogenic pyrene-based tenside, poly(ethylene glycol) pyrenebutanoate, was prepared and applied in capillary isoelectric focusing with fluorometric detection. This dye was used here as a buffer additive in capillary isoelectric focusing for a dynamic modification of the sample of proteins and microorganisms. The values of the isoelectric points of the labeled bioanalytes were calculated with use of the fluorescent pI markers and were found comparable with pI of the native compounds. The mixed cultures of proteins and microorganisms, Escherichia coli CCM 3954, Staphylococcus epidermidis CCM 4418, Proteus vulgaris, Enterococcus faecalis CCM 4224, and Stenotrophomonas maltophilia, the strains of the yeast cells, Candida albicans CCM 8180, Candida krusei, Candida parapsilosis, Candida glabrata, Candida tropicalis, and Saccharomyces cerevisiae were reproducibly focused and separated by the suggested technique. The nonionogenic pyrene-based tenside, poly(ethylene glycol) pyrenebutanoate, was prepared and applied in capillary isoelectric focusing with fluorometric detection. This dye was used here as a buffer additive in capillary isoelectric focusing for a dynamic modification of the sample of proteins and microorganisms. The values of the isoelectric points of the labeled bioanalytes were calculated with use of the fluorescent pI markers and were found comparable with pI of the native compounds. The mixed cultures of proteins and microorganisms, Escherichia coli CCM 3954, Staphylococcus epidermidis CCM 4418, Proteus vulgaris, Enterococcus faecalis CCM 4224, and Stenotrophomonas maltophilia, the strains of the yeast cells, Candida albicans CCM 8180, Candida krusei, Candida parapsilosis, Candida glabrata, Candida tropicalis, and Saccharomyces cerevisiae were reproducibly focused and separated by the suggested technique.
dcterms:title
Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate. Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate.
skos:prefLabel
Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate. Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate.
skos:notation
RIV/00216224:14110/06:00016450!RIV10-MSM-14110___
n3:aktivita
n14:S n14:P n14:Z
n3:aktivity
P(IAA4031302), S, Z(AV0Z40310501)
n3:cisloPeriodika
24
n3:dodaniDat
n8:2010
n3:domaciTvurceVysledku
n4:5900743 n4:2222655 n4:5730155
n3:druhVysledku
n10:J
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
467727
n3:idVysledku
RIV/00216224:14110/06:00016450
n3:jazykVysledku
n17:eng
n3:klicovaSlova
capillary isoelectric focusing; fluorometric detection; proteins; microorganisms; poly(ethylene glycol) pyrenebutanoate.
n3:klicoveSlovo
n9:microorganisms n9:fluorometric%20detection n9:poly%28ethylene%20glycol%29%20pyrenebutanoate. n9:capillary%20isoelectric%20focusing n9:proteins
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[CD917301B0D0]
n3:nazevZdroje
Analytical Chemistry
n3:obor
n18:FN
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
5
n3:projekt
n5:IAA4031302
n3:rokUplatneniVysledku
n8:2006
n3:svazekPeriodika
78
n3:tvurceVysledku
Holá, Veronika Horký, Jaroslav Šlais, Karel Růžička, Filip Horká, Marie
n3:wos
000242824900034
n3:zamer
n16:AV0Z40310501
s:issn
0003-2700
s:numberOfPages
8
n19:organizacniJednotka
14110