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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F06%3A00016448%21RIV10-AV0-14110___
rdf:type
n9:Vysledek skos:Concept
dcterms:description
The isoelectric points of many microbial cells lie within the pH range spanning from 1.5 to 4.5. In this work, we suggest a CIEF method for the separation of cells according to their isoelectric points in the pH range of 2-5. It includes the segmental injection of the sample pulse composed of the segment of the selected simple ampholytes, the segment of the bioanalytes and the segment of carrier ampholytes into fused silica capillaries dynamically modified by poly(ethylene glycole). This polymer dissolved in the catholyte, in the anolyte and in the injected sample pulse was used for a prevention of the bioanalyte adsorption on the capillary surface and for the reduction of the electroosmotic flow. Between each focusing run, the capillaries were washed with the mixture of acetone/ethanol to achieve the reproducible and efficient CIEF. In order to trace of pH gradients, low-molecular-mass pI markers were used. The isoelectric points of many microbial cells lie within the pH range spanning from 1.5 to 4.5. In this work, we suggest a CIEF method for the separation of cells according to their isoelectric points in the pH range of 2-5. It includes the segmental injection of the sample pulse composed of the segment of the selected simple ampholytes, the segment of the bioanalytes and the segment of carrier ampholytes into fused silica capillaries dynamically modified by poly(ethylene glycole). This polymer dissolved in the catholyte, in the anolyte and in the injected sample pulse was used for a prevention of the bioanalyte adsorption on the capillary surface and for the reduction of the electroosmotic flow. Between each focusing run, the capillaries were washed with the mixture of acetone/ethanol to achieve the reproducible and efficient CIEF. In order to trace of pH gradients, low-molecular-mass pI markers were used.
dcterms:title
Capillary isoelectric focusing of microorganisms in the pH range 2-5 in a dynamically modified FS capillary with UV detection. Capillary isoelectric focusing of microorganisms in the pH range 2-5 in a dynamically modified FS capillary with UV detection.
skos:prefLabel
Capillary isoelectric focusing of microorganisms in the pH range 2-5 in a dynamically modified FS capillary with UV detection. Capillary isoelectric focusing of microorganisms in the pH range 2-5 in a dynamically modified FS capillary with UV detection.
skos:notation
RIV/00216224:14110/06:00016448!RIV10-AV0-14110___
n3:aktivita
n15:P n15:Z
n3:aktivity
P(IAA4031302), Z(AV0Z40310501)
n3:cisloPeriodika
5
n3:dodaniDat
n4:2010
n3:domaciTvurceVysledku
n11:5730155 n11:2222655 n11:5900743
n3:druhVysledku
n12:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
467728
n3:idVysledku
RIV/00216224:14110/06:00016448
n3:jazykVysledku
n13:eng
n3:klicovaSlova
capillary isoelectric focusing; microorganisms; Staphylococcus; Candida; Escherichia coli; UV detection.
n3:klicoveSlovo
n8:UV%20detection. n8:Staphylococcus n8:Candida n8:Escherichia%20coli n8:capillary%20isoelectric%20focusing n8:microorganisms
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[422DC13CC015]
n3:nazevZdroje
Analytical and Bioanalytical Chemistry
n3:obor
n19:FN
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:projekt
n17:IAA4031302
n3:rokUplatneniVysledku
n4:2006
n3:svazekPeriodika
385
n3:tvurceVysledku
Holá, Veronika Horká, Marie Šlais, Karel Růžička, Filip
n3:wos
000238496100009
n3:zamer
n14:AV0Z40310501
s:issn
1618-2642
s:numberOfPages
7
n7:organizacniJednotka
14110