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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F06%3A00016448%21RIV08-GA0-14110___
rdf:type
n14:Vysledek skos:Concept
dcterms:description
CIEF v kyselém gradientu pH 2-5 umožňuje separaci a identifikaci mikrobů i ze směsných kultur. CIEF v kyselém gradientu pH 2-5 umožňuje separaci a identifikaci mikrobů i ze směsných kultur. The isoelectric points of many microbial cells lie within the pH range spanning from 1.5 to 4.5. In this work, we suggest a CIEF method for the separation of cells according to their isoelectric points in the pH range of 2-5. It includes the segmental injection of the sample pulse composed of the segment of the selected simple ampholytes, the segment of the bioanalytes and the segment of carrier ampholytes into fused silica capillaries dynamically modified by poly(ethylene glycole). This polymer dissolved in the catholyte, in the anolyte and in the injected sample pulse was used for a prevention of the bioanalyte adsorption on the capillary surface and for the reduction of the electroosmotic flow. Between each focusing run, the capillaries were washed with the mixture of acetone/ethanol to achieve the reproducible and efficient CIEF. In order to trace of pH gradients, low-molecular-mass pI markers were used. The mixed cultures of microorganisms, Escherichia coli CCM 3954, Candida albicans CCM 8180, Ca
dcterms:title
Kapilární isoelektrická fokusace mikroorganismů v pH 2-5 v dynamicky modifikované FS kapiláře s UV detekcí Capillary isoelectric focusing of microorganisms in the pH range 2-5 in a dynamically modified FS capillary with UV detection. Kapilární isoelektrická fokusace mikroorganismů v pH 2-5 v dynamicky modifikované FS kapiláře s UV detekcí
skos:prefLabel
Capillary isoelectric focusing of microorganisms in the pH range 2-5 in a dynamically modified FS capillary with UV detection. Kapilární isoelektrická fokusace mikroorganismů v pH 2-5 v dynamicky modifikované FS kapiláře s UV detekcí Kapilární isoelektrická fokusace mikroorganismů v pH 2-5 v dynamicky modifikované FS kapiláře s UV detekcí
skos:notation
RIV/00216224:14110/06:00016448!RIV08-GA0-14110___
n3:strany
840-846
n3:aktivita
n9:P
n3:aktivity
P(GA301/99/0045)
n3:cisloPeriodika
5
n3:dodaniDat
n11:2008
n3:domaciTvurceVysledku
n10:2222655 n10:5730155 n10:5900743
n3:druhVysledku
n4:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
481423
n3:idVysledku
RIV/00216224:14110/06:00016448
n3:jazykVysledku
n15:cze
n3:klicovaSlova
capillary isoelectric focusing; microorganisms; Staphylococcus; Candida; Escherichia coli; UV detection.
n3:klicoveSlovo
n7:microorganisms n7:Candida n7:Staphylococcus n7:capillary%20isoelectric%20focusing n7:Escherichia%20coli n7:UV%20detection.
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[EFD5FFCF8844]
n3:nazevZdroje
Analytical and Bioanalytical Chemistry
n3:obor
n13:FN
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:projekt
n17:GA301%2F99%2F0045
n3:rokUplatneniVysledku
n11:2006
n3:svazekPeriodika
385
n3:tvurceVysledku
Holá, Veronika Šlais, Karel Růžička, Filip Horká, Marie
s:issn
1618-2642
s:numberOfPages
7
n16:organizacniJednotka
14110