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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F06%3A00015796%21RIV10-GA0-14110___
rdf:type
n13:Vysledek skos:Concept
dcterms:description
1. Several lines of evidence suggest that cytokines and their receptors are initiators of changes in the activity of dorsal root ganglia (DRG) neurons, but their cellular distribution is still very limited or controversial. Therefore, the goal of present study was to investigate immunohistochemical distribution of TNF-a and TNF receptor-1 (TNFR1) proteins in the rat DRG following three types of nerve injury. 2. The unilateral sciatic and spinal nerve ligation as well as the sciatic nerve transection were used to induce changes in the distribution of TNF-a and TNFR1 proteins. The TNF-a and TNFR1 immunofluorescence was assessed in the L4-L5 DRG affected by nerve injury for 1 and 2 weeks, and compared with the contralateral ones and those removed from naive or sham-operated rats. A part of the sections was incubated for simultaneous immunostaining for TNF-a and ED-1. The immunofluorescence brightness was measured by image analysis system (LUCIA-G v4. 1. Several lines of evidence suggest that cytokines and their receptors are initiators of changes in the activity of dorsal root ganglia (DRG) neurons, but their cellular distribution is still very limited or controversial. Therefore, the goal of present study was to investigate immunohistochemical distribution of TNF-a and TNF receptor-1 (TNFR1) proteins in the rat DRG following three types of nerve injury. 2. The unilateral sciatic and spinal nerve ligation as well as the sciatic nerve transection were used to induce changes in the distribution of TNF-a and TNFR1 proteins. The TNF-a and TNFR1 immunofluorescence was assessed in the L4-L5 DRG affected by nerve injury for 1 and 2 weeks, and compared with the contralateral ones and those removed from naive or sham-operated rats. A part of the sections was incubated for simultaneous immunostaining for TNF-a and ED-1. The immunofluorescence brightness was measured by image analysis system (LUCIA-G v4.
dcterms:title
Intra- and Extraneuronal Changes of Immunofluorescence Staining for TNF-a and TNFR1 in the Dorsal Root Ganglia of Rat Peripheral Neuropathic Pain Models Intra- and Extraneuronal Changes of Immunofluorescence Staining for TNF-a and TNFR1 in the Dorsal Root Ganglia of Rat Peripheral Neuropathic Pain Models
skos:prefLabel
Intra- and Extraneuronal Changes of Immunofluorescence Staining for TNF-a and TNFR1 in the Dorsal Root Ganglia of Rat Peripheral Neuropathic Pain Models Intra- and Extraneuronal Changes of Immunofluorescence Staining for TNF-a and TNFR1 in the Dorsal Root Ganglia of Rat Peripheral Neuropathic Pain Models
skos:notation
RIV/00216224:14110/06:00015796!RIV10-GA0-14110___
n3:aktivita
n12:P n12:Z
n3:aktivity
P(GA309/03/1199), Z(MSM0021622404)
n3:cisloPeriodika
4
n3:dodaniDat
n9:2010
n3:domaciTvurceVysledku
n8:8403503 n8:1604074 n8:6754937 n8:5568692 n8:7731361
n3:druhVysledku
n11:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
480238
n3:idVysledku
RIV/00216224:14110/06:00015796
n3:jazykVysledku
n10:eng
n3:klicovaSlova
proinflammatory cytokines; unilateral nerve injury; bilateral reaction
n3:klicoveSlovo
n5:proinflammatory%20cytokines n5:bilateral%20reaction n5:unilateral%20nerve%20injury
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[CB3372D38C69]
n3:nazevZdroje
Cellular and Molecular Neurobiology
n3:obor
n17:FH
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n4:GA309%2F03%2F1199
n3:rokUplatneniVysledku
n9:2006
n3:svazekPeriodika
26
n3:tvurceVysledku
Hradilová Svíženská, Ivana Klusáková, Ilona Jančálek, Radim Pejchalová, Kateřina Dubový, Petr
n3:wos
000242657500011
n3:zamer
n15:MSM0021622404
s:issn
0272-4340
s:numberOfPages
13
n19:organizacniJednotka
14110