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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F05%3A00012467%21RIV11-GA0-14110___
rdf:type
skos:Concept n11:Vysledek
dcterms:description
The cytoskeleton was investigated in Cryptococcus laurentii. The actin cytoskeleton stained by rhodamine-phalloidin in encapsulated strain CCY 17-3-5 failed, but the actin patches were detected by application of monoclonal anti-actin antibody and secondary antibody SwAM-FITC. In acapsular strain C. laurentii 17-3-6, F-actin was stainable by rhodamine-phalloidin. The cortical actin patches were distributed under cell surface and actin cables extended to the bud. At cytokinesis, the actin cables were replaced with contractile ring. Microtubules were seen in both yeast strains. Cytoplasmatic microtubules during interphase arranged into a random network under the plasma membrane. Before mitosis, nucleus migrated from the mother cell to the bud, cytoplasmatic microtubules were disassembled, the spindle formed that allowed the nuclear division. Before the cytokinesis the cytoplasmatic microtubules reappeared. The dynamics of cytoskeleton in C. laurentii is similar to Cryptococcus neoformans (2). The cytoskeleton was investigated in Cryptococcus laurentii. The actin cytoskeleton stained by rhodamine-phalloidin in encapsulated strain CCY 17-3-5 failed, but the actin patches were detected by application of monoclonal anti-actin antibody and secondary antibody SwAM-FITC. In acapsular strain C. laurentii 17-3-6, F-actin was stainable by rhodamine-phalloidin. The cortical actin patches were distributed under cell surface and actin cables extended to the bud. At cytokinesis, the actin cables were replaced with contractile ring. Microtubules were seen in both yeast strains. Cytoplasmatic microtubules during interphase arranged into a random network under the plasma membrane. Before mitosis, nucleus migrated from the mother cell to the bud, cytoplasmatic microtubules were disassembled, the spindle formed that allowed the nuclear division. Before the cytokinesis the cytoplasmatic microtubules reappeared. The dynamics of cytoskeleton in C. laurentii is similar to Cryptococcus neoformans (2).
dcterms:title
Cytoskeleton during the cell division cycle of the yeast Cryptococcus laurentii Cytoskeleton during the cell division cycle of the yeast Cryptococcus laurentii
skos:prefLabel
Cytoskeleton during the cell division cycle of the yeast Cryptococcus laurentii Cytoskeleton during the cell division cycle of the yeast Cryptococcus laurentii
skos:notation
RIV/00216224:14110/05:00012467!RIV11-GA0-14110___
n3:aktivita
n17:P
n3:aktivity
P(GA310/03/1195)
n3:dodaniDat
n7:2011
n3:domaciTvurceVysledku
n4:3299562 n4:8883769 n4:3225623
n3:druhVysledku
n10:O
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
516761
n3:idVysledku
RIV/00216224:14110/05:00012467
n3:jazykVysledku
n8:eng
n3:klicovaSlova
basidiomycetous yeast; Cryptococcus laurentii; cytoskeleton; F-actin; microtubules
n3:klicoveSlovo
n5:F-actin n5:microtubules n5:Cryptococcus%20laurentii n5:basidiomycetous%20yeast n5:cytoskeleton
n3:kontrolniKodProRIV
[FFD592B46618]
n3:obor
n16:FP
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n12:GA310%2F03%2F1195
n3:rokUplatneniVysledku
n7:2005
n3:tvurceVysledku
Gabriel, Miroslav Kopecká, Marie David, Marek
n15:organizacniJednotka
14110