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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F04%3A00011578%21RIV08-GA0-14110___
rdf:type
n15:Vysledek skos:Concept
dcterms:description
Sigma receptor ligand haloperidol je psychotropní látka užívaná v léčbě různých psychiatrických onemocnění. Byly popsány těžké vedlejší účinky na kardiovaskulární systém (hlavně komorové arythmie). zkoumali jsme účinek 10 microM haloperidolu na srdeční iontové proudy u dvou modelů -izolovaných kardiomyocytů potkana a HEK293 buněk s přechodně exprimovaným CaV1.2 L-typem kalciového kanálu. Exprese sigma receptorů v obou modelech byla měřena RT-PCR. Primery were získány ze sekvence sigma receptorů (potkan-Genbank number 38541100, lidské-Genbank number 22212933) a amplifikovány 185bp fragmentem v pozici 499 - 684 nt. Metodou whole cell patch-clamp jsme zjišťovali účinek haloperidolu na sodíková, draslíkové a kalciový proudy. V kardiomyocytech potkana haloperidol inhiboval 95% INa aktivovaného 40 ms napěťovými pulzy z 75 mV do 20 mV. Inhibice Ito a IK,end byla měřena 300 ms napěťovými pulzy z 75 mV do +40 mV. Byla pozorována 80% inhibice Ito a 37% inhibice IK,end, vždy reverzibilní. Inaktivace Ito byla hal Sigma receptor ligand haloperidol is a psychotropic drug used in the treatment of various psychiatric disorders. Severe cardiovascular side effects (mostly ventricular arrhythmias) have been often reported as a consequence of this treatment. Thus, we have investigated the effects of 10 ľM haloperidol on cardiac ionic currents in two models freshly isolated rat ventricular cardiomyocytes and in HEK 293 cells with transiently expressed CaV1.2 L-type calcium channel. Gene expression of sigma receptors in both models was measured by RT-PCR. Primers were designed from sequence of sigma receptors (for rat -Genbank number 38541100, for human Genbank number 22212933) and amplified 185bp fragment in the position 499 - 684 nt. The whole cell patch-clamp method has been employed to assess the effect of haloperidol on sodium, potassium and calcium currents. In rat cardiac cells, haloperidol inhibited 95% of INa activated by 40 ms voltage pulses from 75 mV to 20 mV. The inhibition of Ito and IK,end were measured b Sigma receptor ligand haloperidol is a psychotropic drug used in the treatment of various psychiatric disorders. Severe cardiovascular side effects (mostly ventricular arrhythmias) have been often reported as a consequence of this treatment. Thus, we have investigated the effects of 10 ľM haloperidol on cardiac ionic currents in two models freshly isolated rat ventricular cardiomyocytes and in HEK 293 cells with transiently expressed CaV1.2 L-type calcium channel. Gene expression of sigma receptors in both models was measured by RT-PCR. Primers were designed from sequence of sigma receptors (for rat -Genbank number 38541100, for human Genbank number 22212933) and amplified 185bp fragment in the position 499 - 684 nt. The whole cell patch-clamp method has been employed to assess the effect of haloperidol on sodium, potassium and calcium currents. In rat cardiac cells, haloperidol inhibited 95% of INa activated by 40 ms voltage pulses from 75 mV to 20 mV. The inhibition of Ito and IK,end were measured b
dcterms:title
Effect of Sigma Ligand Haloperidol on Cardiac Excitability Účinek sigma ligandu haloperidolu na srdeční excitabilitu Effect of Sigma Ligand Haloperidol on Cardiac Excitability
skos:prefLabel
Effect of Sigma Ligand Haloperidol on Cardiac Excitability Účinek sigma ligandu haloperidolu na srdeční excitabilitu Effect of Sigma Ligand Haloperidol on Cardiac Excitability
skos:notation
RIV/00216224:14110/04:00011578!RIV08-GA0-14110___
n3:strany
33
n3:aktivita
n11:P
n3:aktivity
P(GA305/04/1385)
n3:dodaniDat
n10:2008
n3:domaciTvurceVysledku
n4:8165459 n4:7511671 n4:1457160 n4:1469517
n3:druhVysledku
n16:D
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
562004
n3:idVysledku
RIV/00216224:14110/04:00011578
n3:jazykVysledku
n13:eng
n3:klicovaSlova
cardiac excitability; sigma receptor; haloperidol; ionic currents; inhibition
n3:klicoveSlovo
n6:sigma%20receptor n6:ionic%20currents n6:haloperidol n6:cardiac%20excitability n6:inhibition
n3:kontrolniKodProRIV
[AA6058FC10CF]
n3:mistoKonaniAkce
La Grande-Motte
n3:mistoVydani
Montpellier, Francie
n3:nazevZdroje
New Frontiers in Basic Cardiovascular Research
n3:obor
n18:ED
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
7
n3:projekt
n20:GA305%2F04%2F1385
n3:rokUplatneniVysledku
n10:2004
n3:tvurceVysledku
Tarabová, Bohuslava Križanová, Olga Nováková, Marie Matejovič, Peter Bébarová, Markéta Lacinová, Lubica Pásek, Michal
n3:typAkce
n5:EUR
n3:zahajeniAkce
2004-09-24+02:00
s:numberOfPages
1
n12:hasPublisher
INSERM
n7:organizacniJednotka
14110