This HTML5 document contains 44 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n18http://localhost/temp/predkladatel/
n10http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n7http://linked.opendata.cz/resource/domain/vavai/projekt/
n11http://linked.opendata.cz/ontology/domain/vavai/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n16http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216224%3A14110%2F03%3A00012312%21RIV%2F2005%2FMZ0%2F141105%2FN/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n14http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n13http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n12http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n6http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n15http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F03%3A00012312%21RIV%2F2005%2FMZ0%2F141105%2FN
rdf:type
n11:Vysledek skos:Concept
dcterms:description
Byly vyšetřeny kmeny Treponema pallidum subsp. pallidum (původce syfilis) a T. pallidum subsp. pertenue (původce yaws) pomocí DNA-microarray techniky a genomového sekvenování. Zjištěné rozdíly byly diskutovány v kontextu současných poznatků. DNA of Treponema pallidum subsp. pallidum Nichols strain was compared to DNA isolated from three different strains of T. pallidum subsp. pertenue (strain Gauthier, Samoan D, CDC-2). As a result of DNA microarray comparisons, 25 genes (13 with stronger and 6 with weaker signal in pertenue strains and 6 control genes with similar signal in both subspecies examined) were selected and sequenced. Altogether, 24083 nucleotides (2.12% of the genome) were sequenced in 3 pertenue strains and control Nichols. No region of extensive sequence heterogeneity was detected. However, 15 different single nucleotide polymorfisms (SNP) were identified: 3 SNPs in Gauthier strain, 14 in Samoan D and 15 SNPs in CDC-2. Ten (out of 15) SNPs cause amino acid changes. SNPs common for all pertenue strains as well as SNPs specific for each individual strain will allow to use these nucleotide polymorfisms to design sequence specific PCR diagnostics of these strains. DNA of Treponema pallidum subsp. pallidum Nichols strain was compared to DNA isolated from three different strains of T. pallidum subsp. pertenue (strain Gauthier, Samoan D, CDC-2). As a result of DNA microarray comparisons, 25 genes (13 with stronger and 6 with weaker signal in pertenue strains and 6 control genes with similar signal in both subspecies examined) were selected and sequenced. Altogether, 24083 nucleotides (2.12% of the genome) were sequenced in 3 pertenue strains and control Nichols. No region of extensive sequence heterogeneity was detected. However, 15 different single nucleotide polymorfisms (SNP) were identified: 3 SNPs in Gauthier strain, 14 in Samoan D and 15 SNPs in CDC-2. Ten (out of 15) SNPs cause amino acid changes. SNPs common for all pertenue strains as well as SNPs specific for each individual strain will allow to use these nucleotide polymorfisms to design sequence specific PCR diagnostics of these strains.
dcterms:title
Komparativní genomika patogenních poddruhů Treponema pallidum Comparative genomics of pathogenic Treponema pallidum subspecies Comparative genomics of pathogenic Treponema pallidum subspecies
skos:prefLabel
Komparativní genomika patogenních poddruhů Treponema pallidum Comparative genomics of pathogenic Treponema pallidum subspecies Comparative genomics of pathogenic Treponema pallidum subspecies
skos:notation
RIV/00216224:14110/03:00012312!RIV/2005/MZ0/141105/N
n3:strany
297-298
n3:aktivita
n9:P
n3:aktivity
P(NI7351)
n3:cisloPeriodika
97
n3:dodaniDat
n15:2005
n3:domaciTvurceVysledku
n10:9995307 n10:4942558
n3:druhVysledku
n6:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
601652
n3:idVysledku
RIV/00216224:14110/03:00012312
n3:jazykVysledku
n13:eng
n3:klicovaSlova
comparative genomics, Treponema pallidum
n3:klicoveSlovo
n14:comparative%20genomics n14:Treponema%20pallidum
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[0C705A093CD4]
n3:nazevZdroje
CHEMICKÉ LISTY
n3:obor
n12:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n7:NI7351
n3:rokUplatneniVysledku
n15:2003
n3:svazekPeriodika
2003
n3:tvurceVysledku
Norris, S. J. Weinstock, G. M. Matějková, Petra Šmajs, David
s:issn
0009-2770
s:numberOfPages
2
n18:organizacniJednotka
14110