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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F03%3A00008552%21RIV08-MSM-14110___
rdf:type
skos:Concept n12:Vysledek
dcterms:description
Dendritic cells (DCs) are antigen-presenting cells that play a critical role in the induction of cytotoxic T-lymphocytes. An optimal method for the generation of DC for clinical use remains to be established. The aim of our study was to find an optimal cytokine combination for DC generation from peripheral blood stem cells (PBSC) and peripheral blood mononuclear cells (PBMC) in serum-free conditions. Serial immunophenotyping enabled us to observe changes in DC content during the culture as well as the development of maturation and activation markers. As a source for DC culture, we used PBSC from patients with multiple myeloma after stem cell mobilization using cyclophosphamide and G-CSF, or PBMC from healthy donors without mobilization. The cells were cultured in a serum-free medium with different cytokine combinations including GM-CSF, TNF-[alpha], Flt-3, CD40L, IFN-[gamma], IL-1[alpha], IL-6, PGE1, and IL-4. The cell cultures were evaluated by immunophenotyping. For PBMC, interleukin-12 assay was pe Dendritic cells (DCs) are antigen-presenting cells that play a critical role in the induction of cytotoxic T-lymphocytes. An optimal method for the generation of DC for clinical use remains to be established. The aim of our study was to find an optimal cytokine combination for DC generation from peripheral blood stem cells (PBSC) and peripheral blood mononuclear cells (PBMC) in serum-free conditions. Serial immunophenotyping enabled us to observe changes in DC content during the culture as well as the development of maturation and activation markers. As a source for DC culture, we used PBSC from patients with multiple myeloma after stem cell mobilization using cyclophosphamide and G-CSF, or PBMC from healthy donors without mobilization. The cells were cultured in a serum-free medium with different cytokine combinations including GM-CSF, TNF-[alpha], Flt-3, CD40L, IFN-[gamma], IL-1[alpha], IL-6, PGE1, and IL-4. The cell cultures were evaluated by immunophenotyping. For PBMC, interleukin-12 assay was pe Dendritic cells (DCs) are antigen-presenting cells that play a critical role in the induction of cytotoxic T-lymphocytes. An optimal method for the generation of DC for clinical use remains to be established. The aim of our study was to find an optimal cytokine combination for DC generation from peripheral blood stem cells (PBSC) and peripheral blood mononuclear cells (PBMC) in serum-free conditions. Serial immunophenotyping enabled us to observe changes in DC content during the culture as well as the development of maturation and activation markers. As a source for DC culture, we used PBSC from patients with multiple myeloma after stem cell mobilization using cyclophosphamide and G-CSF, or PBMC from healthy donors without mobilization. The cells were cultured in a serum-free medium with different cytokine combinations including GM-CSF, TNF-[alpha], Flt-3, CD40L, IFN-[gamma], IL-1[alpha], IL-6, PGE1, and IL-4. The cell cultures were evaluated by immunophenotyping. For PBMC, interleukin-12 assay was pe
dcterms:title
Generation of antigen-loaded dendritic cells in a serum-free medium using different cytokine combinations. Generation of antigen-loaded dendritic cells in a serum-free medium using different cytokine combinations. Generation of antigen-loaded dendritic cells in a serum-free medium using different cytokine combinations.
skos:prefLabel
Generation of antigen-loaded dendritic cells in a serum-free medium using different cytokine combinations. Generation of antigen-loaded dendritic cells in a serum-free medium using different cytokine combinations. Generation of antigen-loaded dendritic cells in a serum-free medium using different cytokine combinations.
skos:notation
RIV/00216224:14110/03:00008552!RIV08-MSM-14110___
n3:strany
877-882
n3:aktivita
n4:P n4:Z
n3:aktivity
P(NC6152), P(NC6763), Z(MSM 141100003)
n3:cisloPeriodika
2/2003
n3:dodaniDat
n14:2008
n3:domaciTvurceVysledku
n11:9622748
n3:druhVysledku
n7:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
608098
n3:idVysledku
RIV/00216224:14110/03:00008552
n3:jazykVysledku
n6:eng
n3:klicovaSlova
dendritic cells; in vitro cell culture; cytokines; immunotherapy
n3:klicoveSlovo
n5:cytokines n5:immunotherapy n5:dendritic%20cells n5:in%20vitro%20cell%20culture
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[FEC8771A2D0D]
n3:nazevZdroje
Vaccine
n3:obor
n13:FD
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
14
n3:projekt
n16:NC6763 n16:NC6152
n3:rokUplatneniVysledku
n14:2003
n3:svazekPeriodika
neuveden
n3:tvurceVysledku
Hájek, Roman Doubek, M. Mareschová, Iveta Penka, Miroslav Svobodník, A. Vorlíček, Jiří Vidláková, Petra Bourková, Ludmila Buliková, Alena Kovářová, Lucie Váňová, Pavlína Büchler, Tomáš Tůzová, Eva Musilová, Romana
n3:zamer
n18:MSM%20141100003
s:issn
0264-410X
s:numberOfPages
6
n15:organizacniJednotka
14110