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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F03%3A00008399%21RIV09-GA0-14110___
rdf:type
n15:Vysledek skos:Concept
dcterms:description
Spectrin, a component of the membrane skeleton in erythrocytes and other animal cells, has also been identified in plant and fungal cells. However, its postulated role, i.e., the maintenance of shape and elasticity of the plasma membrane, is probably not exerted in walled cells. To study spectrin in these cells, we chose yeasts because of a high morphological variability of their life cycle. The localization of spectrin in the cells and protoplasts of Saccharomyces cerevisiae and Schizosaccharomyces japonicus var. versatilis was detected by Western blotting, indirect immunofluorescence and immunogold electron microscopy techniques with the use of anti-chicken and anti-human erythrocyte spectrin antibodies. A 220/240 kDa protein band and some bands of lower molecular weights were detected in cell and protoplast extracts of both yeast strains. Spectrin-like proteins were revealed by fluorescence microscopy at cell surfaces and in vacuolar membranes. Immunogold-labelling showed spectrin-like proteins in Spectrin, a component of the membrane skeleton in erythrocytes and other animal cells, has also been identified in plant and fungal cells. However, its postulated role, i.e., the maintenance of shape and elasticity of the plasma membrane, is probably not exerted in walled cells. To study spectrin in these cells, we chose yeasts because of a high morphological variability of their life cycle. The localization of spectrin in the cells and protoplasts of Saccharomyces cerevisiae and Schizosaccharomyces japonicus var. versatilis was detected by Western blotting, indirect immunofluorescence and immunogold electron microscopy techniques with the use of anti-chicken and anti-human erythrocyte spectrin antibodies. A 220/240 kDa protein band and some bands of lower molecular weights were detected in cell and protoplast extracts of both yeast strains. Spectrin-like proteins were revealed by fluorescence microscopy at cell surfaces and in vacuolar membranes. Immunogold-labelling showed spectrin-like proteins in Spectrin, a component of the membrane skeleton in erythrocytes and other animal cells, has also been identified in plant and fungal cells. However, its postulated role, i.e., the maintenance of shape and elasticity of the plasma membrane, is probably not exerted in walled cells. To study spectrin in these cells, we chose yeasts because of a high morphological variability of their life cycle. The localization of spectrin in the cells and protoplasts of Saccharomyces cerevisiae and Schizosaccharomyces japonicus var. versatilis was detected by Western blotting, indirect immunofluorescence and immunogold electron microscopy techniques with the use of anti-chicken and anti-human erythrocyte spectrin antibodies. A 220/240 kDa protein band and some bands of lower molecular weights were detected in cell and protoplast extracts of both yeast strains. Spectrin-like proteins were revealed by fluorescence microscopy at cell surfaces and in vacuolar membranes. Immunogold-labelling showed spectrin-like proteins in
dcterms:title
Immunodetection of Spectrin-like Proteins in Yeasts Immunodetection of Spectrin-like Proteins in Yeasts Immunodetection of Spectrin-like Proteins in Yeasts
skos:prefLabel
Immunodetection of Spectrin-like Proteins in Yeasts Immunodetection of Spectrin-like Proteins in Yeasts Immunodetection of Spectrin-like Proteins in Yeasts
skos:notation
RIV/00216224:14110/03:00008399!RIV09-GA0-14110___
n3:aktivita
n16:P
n3:aktivity
P(GA204/00/0394), P(GA204/99/D025)
n3:cisloPeriodika
3
n3:dodaniDat
n10:2009
n3:domaciTvurceVysledku
n7:5751055 n7:9540539 n7:6317804
n3:druhVysledku
n17:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
609828
n3:idVysledku
RIV/00216224:14110/03:00008399
n3:jazykVysledku
n14:eng
n3:klicovaSlova
spectrin; yeast; cytoskeleton; ELM; Saccharomyces; membrane skeleton
n3:klicoveSlovo
n9:spectrin n9:cytoskeleton n9:ELM n9:Saccharomyces n9:membrane%20skeleton n9:yeast
n3:kodStatuVydavatele
CA - Kanada
n3:kontrolniKodProRIV
[99167A1800BF]
n3:nazevZdroje
Canadian Journal of Microbiology
n3:obor
n4:EE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n8:GA204%2F99%2FD025 n8:GA204%2F00%2F0394
n3:rokUplatneniVysledku
n10:2003
n3:svazekPeriodika
49
n3:tvurceVysledku
Svoboda, Augustin Slaninová, Iva Holubářová, Alena
n3:wos
000183010400005
s:issn
0008-4166
s:numberOfPages
8
n11:organizacniJednotka
14110