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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F02%3A00007250%21RIV%2F2003%2FMSM%2F141103%2FN
rdf:type
skos:Concept n15:Vysledek
dcterms:description
We previously reported that therapy of human cervical carcinoma HeLa cells with CP induced segregation of nucleoli and changes of nuclei characteristic of apoptosis. We raised the question of whether p53 can be reactivated by chemotherapy in HeLa cells despite the presence of HPV-encoded E6 activity. Since the reduced stability of wild-type p53 protein in HeLa cells is a consequence of its enhanced ubiquitination by virally encoded E6 protein, resulting in its accelerated degradation, we checked the cellular level of E6 during CP therapy. Six hours after application of CP, E6 protein expression was markedly reduced. This coincided with the increase of cellular p53 and preceded the nucleolar accumulation of p53 protein, indicating that repression of virally coded E6 protein by CP contributes to the restoration of p53 expression. We previously reported that therapy of human cervical carcinoma HeLa cells with CP induced segregation of nucleoli and changes of nuclei characteristic of apoptosis. We raised the question of whether p53 can be reactivated by chemotherapy in HeLa cells despite the presence of HPV-encoded E6 activity. Since the reduced stability of wild-type p53 protein in HeLa cells is a consequence of its enhanced ubiquitination by virally encoded E6 protein, resulting in its accelerated degradation, we checked the cellular level of E6 during CP therapy. Six hours after application of CP, E6 protein expression was markedly reduced. This coincided with the increase of cellular p53 and preceded the nucleolar accumulation of p53 protein, indicating that repression of virally coded E6 protein by CP contributes to the restoration of p53 expression.
dcterms:title
Escape of p53 protein from E6-mediated degradation in HeLa cells after cisplatin therapy Escape of p53 protein from E6-mediated degradation in HeLa cells after cisplatin therapy
skos:prefLabel
Escape of p53 protein from E6-mediated degradation in HeLa cells after cisplatin therapy Escape of p53 protein from E6-mediated degradation in HeLa cells after cisplatin therapy
skos:notation
RIV/00216224:14110/02:00007250!RIV/2003/MSM/141103/N
n4:strany
128
n4:aktivita
n13:Z
n4:aktivity
Z(MSM 141100002)
n4:cisloPeriodika
2
n4:dodaniDat
n11:2003
n4:domaciTvurceVysledku
n8:9787968 n8:5075149
n4:druhVysledku
n16:J
n4:duvernostUdaju
n12:S
n4:entitaPredkladatele
n14:predkladatel
n4:idSjednocenehoVysledku
645258
n4:idVysledku
RIV/00216224:14110/02:00007250
n4:jazykVysledku
n6:eng
n4:klicovaSlova
apoptosis, nucleolar segregation, E6, cisplatin, PARP-1
n4:klicoveSlovo
n5:apoptosis n5:E6 n5:cisplatin n5:PARP-1 n5:nucleolar%20segregation
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[637524857387]
n4:nazevZdroje
International Journal of Cancer
n4:obor
n18:EB
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
4
n4:pocetUcastnikuAkce
0
n4:pocetZahranicnichUcastnikuAkce
0
n4:rokUplatneniVysledku
n11:2002
n4:svazekPeriodika
101
n4:tvurceVysledku
Horký, Marcel Kotala, Vladimír Wesierska-Gadek, Jozefa Schloffer, Daniela
n4:zamer
n10:MSM%20141100002
s:issn
0020-7136
s:numberOfPages
8
n17:organizacniJednotka
14110