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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F02%3A00006732%21RIV08-GA0-14110___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
Proteins related to the a- and b- spectrin have been detected in almost all vertebrate tissues, and also in plants (1) and hyphal fungae (2). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies detected band at 220 kDa in cells and protoplasts extracts of both yeast strains. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast species. In addition, the antigens were spread in the cytoplasm of both cells as fluorescent patches and as a diffuse fluorescence in Sch. versatilis nuclei. In mating and sporulating cells spectrin-like antigens lined the cell surface and prespore membranes. Electron microscopy of Proteins related to the a- and b- spectrin have been detected in almost all vertebrate tissues, and also in plants (1) and hyphal fungae (2). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies detected band at 220 kDa in cells and protoplasts extracts of both yeast strains. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast species. In addition, the antigens were spread in the cytoplasm of both cells as fluorescent patches and as a diffuse fluorescence in Sch. versatilis nuclei. In mating and sporulating cells spectrin-like antigens lined the cell surface and prespore membranes. Electron microscopy of Proteins related to the a- and b- spectrin have been detected in almost all vertebrate tissues, and also in plants (1) and hyphal fungae (2). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies detected band at 220 kDa in cells and protoplasts extracts of both yeast strains. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast species. In addition, the antigens were spread in the cytoplasm of both cells as fluorescent patches and as a diffuse fluorescence in Sch. versatilis nuclei. In mating and sporulating cells spectrin-like antigens lined the cell surface and prespore membranes. Electron microscopy of
dcterms:title
Detection of spectrin-like protein in yeasts: spectrin-actin relations Detection of spectrin-like protein in yeasts: spectrin-actin relations Detection of spectrin-like protein in yeasts: spectrin-actin relations
skos:prefLabel
Detection of spectrin-like protein in yeasts: spectrin-actin relations Detection of spectrin-like protein in yeasts: spectrin-actin relations Detection of spectrin-like protein in yeasts: spectrin-actin relations
skos:notation
RIV/00216224:14110/02:00006732!RIV08-GA0-14110___
n3:strany
14
n3:aktivita
n19:P
n3:aktivity
P(GA204/00/0394), P(GA204/99/D025)
n3:dodaniDat
n9:2008
n3:domaciTvurceVysledku
n12:6317804 n12:9540539 n12:5751055
n3:druhVysledku
n14:D
n3:duvernostUdaju
n20:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
642648
n3:idVysledku
RIV/00216224:14110/02:00006732
n3:jazykVysledku
n17:eng
n3:klicovaSlova
yeast; spectrin; cytoskeleton; actin
n3:klicoveSlovo
n7:yeast n7:actin n7:cytoskeleton n7:spectrin
n3:kontrolniKodProRIV
[88DCE943F4C8]
n3:mistoKonaniAkce
Vranovská Ves
n3:mistoVydani
Praha
n3:nazevZdroje
Sborník X. Cytoskeletálního klubu
n3:obor
n16:EE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n10:GA204%2F99%2FD025 n10:GA204%2F00%2F0394
n3:rokUplatneniVysledku
n9:2002
n3:tvurceVysledku
Holubářová, Alena Slaninová, Iva Svoboda, Augustin
n3:typAkce
n4:CST
n3:zahajeniAkce
2002-04-24+02:00
s:numberOfPages
1
n13:hasPublisher
Československá biologická společnost
n5:organizacniJednotka
14110