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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F01%3A00005112%21RIV%2F2002%2FGA0%2F141102%2FN
rdf:type
n17:Vysledek skos:Concept
dcterms:description
The actin cables and microtubules were disrupted during protoplasting in Saccharomyces cerevisiae cells. In the process of protoplast regeneration, the cytoplasmic microtubules were the first to be restored; actin patches remained regularly distributed under the surface of growing protoplast. After the cell wall had been regenerated in a gelatine medium, actin patches aggregated into clusters, which marked the site of bud development. An incomplete cell wall was the apparent cause for uncoupling between karyokinesis and cytokinesis The presence of several nuclei in the cell gave rise to several buds emerging simultaneously and was probably related to their random positions. In haploids, however, the axial type of budding was obvious also in the reverting protoplasts. Our observations suggest that actin is a structure involved in the restoration of polar growth during protoplast regeneration and that the cell wall plays an important role in this process: in its absence, actin fails to polarise. The actin cables and microtubules were disrupted during protoplasting in Saccharomyces cerevisiae cells. In the process of protoplast regeneration, the cytoplasmic microtubules were the first to be restored; actin patches remained regularly distributed under the surface of growing protoplast. After the cell wall had been regenerated in a gelatine medium, actin patches aggregated into clusters, which marked the site of bud development. An incomplete cell wall was the apparent cause for uncoupling between karyokinesis and cytokinesis The presence of several nuclei in the cell gave rise to several buds emerging simultaneously and was probably related to their random positions. In haploids, however, the axial type of budding was obvious also in the reverting protoplasts. Our observations suggest that actin is a structure involved in the restoration of polar growth during protoplast regeneration and that the cell wall plays an important role in this process: in its absence, actin fails to polarise.
dcterms:title
Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae
skos:prefLabel
Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae
skos:notation
RIV/00216224:14110/01:00005112!RIV/2002/GA0/141102/N
n3:strany
325
n3:aktivita
n15:P
n3:aktivity
P(GA204/00/0394), P(GA204/99/D025)
n3:cisloPeriodika
2
n3:dodaniDat
n4:2002
n3:domaciTvurceVysledku
n6:5751055 n6:6317804 n6:9540539
n3:druhVysledku
n10:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
676755
n3:idVysledku
RIV/00216224:14110/01:00005112
n3:jazykVysledku
n16:eng
n3:klicovaSlova
yeast, protoplasts, cell wall, actin, microtubules, cell polarity
n3:klicoveSlovo
n5:cell%20wall n5:protoplasts n5:microtubules n5:cell%20polarity n5:actin n5:yeast
n3:kodStatuVydavatele
HU - Maďarsko
n3:kontrolniKodProRIV
[95882DBA72C6]
n3:nazevZdroje
Acta Biologica Hungarica
n3:obor
n11:EA
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n7:GA204%2F99%2FD025 n7:GA204%2F00%2F0394
n3:rokUplatneniVysledku
n4:2001
n3:svazekPeriodika
52
n3:tvurceVysledku
Slaninová, Iva Svoboda, Augustin Holubářová, Alena
s:issn
0236-5383
s:numberOfPages
8
n12:organizacniJednotka
14110