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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F01%3A00004307%21RIV09-GA0-14110___
rdf:type
skos:Concept n12:Vysledek
dcterms:description
We compared the effects of four quaternary benzo[c]phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine, and two quaternary protoberberine alkaloids, berberine and coptisine, on the human cell line HeLa (cervix carcinoma cells) and the yeasts Saccharomyces cerevisiae and Schizosaccharomyces japonicus var. versatilis. The ability of alkaloids to display primary fluorescence, allowed us to record their dynamics and localization in cells. Cytotoxicity, anti-microtubular and anti-actin effects in living cells were studied. In the yeasts, neither microtubules nor cell growth were seriously affected even at the alkaloid concentration of 100 mg/mL. The HeLa cells, however, responded to the toxic effect of alkaloids at concentrations ranging from 1-50 mg/mL. IC50 values for individual alkaloids were: sanguinarine IC50 = 0.8 mg/mL, sanguilutine IC50 = 8.3 mg/mL, chelerythrine IC50 = 6.2 mg/mL, chelilutine IC50 = 5.2 mg/mL, coptisine IC50 = 2.6 mg/mL and berberine IC50 =10.0 We compared the effects of four quaternary benzo[c]phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine, and two quaternary protoberberine alkaloids, berberine and coptisine, on the human cell line HeLa (cervix carcinoma cells) and the yeasts Saccharomyces cerevisiae and Schizosaccharomyces japonicus var. versatilis. The ability of alkaloids to display primary fluorescence, allowed us to record their dynamics and localization in cells. Cytotoxicity, anti-microtubular and anti-actin effects in living cells were studied. In the yeasts, neither microtubules nor cell growth were seriously affected even at the alkaloid concentration of 100 mg/mL. The HeLa cells, however, responded to the toxic effect of alkaloids at concentrations ranging from 1-50 mg/mL. IC50 values for individual alkaloids were: sanguinarine IC50 = 0.8 mg/mL, sanguilutine IC50 = 8.3 mg/mL, chelerythrine IC50 = 6.2 mg/mL, chelilutine IC50 = 5.2 mg/mL, coptisine IC50 = 2.6 mg/mL and berberine IC50 =10.0 We compared the effects of four quaternary benzo[c]phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine, and two quaternary protoberberine alkaloids, berberine and coptisine, on the human cell line HeLa (cervix carcinoma cells) and the yeasts Saccharomyces cerevisiae and Schizosaccharomyces japonicus var. versatilis. The ability of alkaloids to display primary fluorescence, allowed us to record their dynamics and localization in cells. Cytotoxicity, anti-microtubular and anti-actin effects in living cells were studied. In the yeasts, neither microtubules nor cell growth were seriously affected even at the alkaloid concentration of 100 mg/mL. The HeLa cells, however, responded to the toxic effect of alkaloids at concentrations ranging from 1-50 mg/mL. IC50 values for individual alkaloids were: sanguinarine IC50 = 0.8 mg/mL, sanguilutine IC50 = 8.3 mg/mL, chelerythrine IC50 = 6.2 mg/mL, chelilutine IC50 = 5.2 mg/mL, coptisine IC50 = 2.6 mg/mL and berberine IC50 =10.0
dcterms:title
Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells
skos:prefLabel
Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells
skos:notation
RIV/00216224:14110/01:00004307!RIV09-GA0-14110___
n3:aktivita
n18:P
n3:aktivity
P(GA204/99/D025)
n3:cisloPeriodika
1
n3:dodaniDat
n8:2009
n3:domaciTvurceVysledku
n10:3682455 n10:2115549 n10:9540539 n10:9382925
n3:druhVysledku
n4:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n5:predkladatel
n3:idSjednocenehoVysledku
683212
n3:idVysledku
RIV/00216224:14110/01:00004307
n3:jazykVysledku
n9:eng
n3:klicovaSlova
benzo[c]phenanthridine; protoberberine alkaloids; cytotoxicity; yeast; cytoskeleton; microtubules
n3:klicoveSlovo
n11:microtubules n11:protoberberine%20alkaloids n11:benzo%5Bc%5Dphenanthridine n11:yeast n11:cytoskeleton n11:cytotoxicity
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[A0771F84BFF4]
n3:nazevZdroje
Cell Biology and Toxicology
n3:obor
n13:EA
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n16:GA204%2F99%2FD025
n3:rokUplatneniVysledku
n8:2001
n3:svazekPeriodika
17
n3:tvurceVysledku
Bochořáková, Hana Slanina, Jiří Slaninová, Iva Táborská, Eva
n3:wos
000169125300006
s:issn
0742-2091
s:numberOfPages
13
n17:organizacniJednotka
14110