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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F00%3A00005108%21RIV%2F2002%2FGA0%2F141102%2FN
rdf:type
skos:Concept n17:Vysledek
dcterms:description
The cells of Saccharomyces cerevisiae were exposed to the effect of mild and lethal heat stress and their viability and changes in their microtubular and actin structures were studied. An increase in cultivation temperature from 25 C to 46 C (lethal heat shock, LHS) resulted, during 10 min, in a rapid decrease in cell viability. However, a mild increase in incubation temperature from 25 C to 37 C or 41 C (mild heat shock, MHS) induced cell tolerance to LHS and the cells were able to survive at 46 C for up to 30 min. A mild heat shock increased the level of Hsp104 in cells, as demonstrated by western blot analysis and indirect immunofluorescence microscopy. In cell exposed to LHS, immunofluorescence microscopy showed rapid degradation of both cytoplasmic and nuclear microtubules, but the spindle pole bodies (SPB) remained preserved. On the other hand, MHS did not produce microtubule degradation. Microtubules remained stable after the LHS that had been preceded by cell cultivation at 37 C. It is sug The cells of Saccharomyces cerevisiae were exposed to the effect of mild and lethal heat stress and their viability and changes in their microtubular and actin structures were studied. An increase in cultivation temperature from 25 C to 46 C (lethal heat shock, LHS) resulted, during 10 min, in a rapid decrease in cell viability. However, a mild increase in incubation temperature from 25 C to 37 C or 41 C (mild heat shock, MHS) induced cell tolerance to LHS and the cells were able to survive at 46 C for up to 30 min. A mild heat shock increased the level of Hsp104 in cells, as demonstrated by western blot analysis and indirect immunofluorescence microscopy. In cell exposed to LHS, immunofluorescence microscopy showed rapid degradation of both cytoplasmic and nuclear microtubules, but the spindle pole bodies (SPB) remained preserved. On the other hand, MHS did not produce microtubule degradation. Microtubules remained stable after the LHS that had been preceded by cell cultivation at 37 C. It is sug
dcterms:title
A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures
skos:prefLabel
A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures
skos:notation
RIV/00216224:14110/00:00005108!RIV/2002/GA0/141102/N
n3:strany
381
n3:aktivita
n14:P
n3:aktivity
P(GA204/00/0394)
n3:cisloPeriodika
6
n3:dodaniDat
n8:2002
n3:domaciTvurceVysledku
n13:5751055 n13:6317804 n13:5395488
n3:druhVysledku
n11:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
703691
n3:idVysledku
RIV/00216224:14110/00:00005108
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Saccharomyces cerevisiae, heat shock, Hsp104, actin, microtubules
n3:klicoveSlovo
n6:actin n6:microtubules n6:heat%20shock n6:Hsp104 n6:Saccharomyces%20cerevisiae
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[0321594EAD07]
n3:nazevZdroje
Scripta medica
n3:obor
n16:EA
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n4:GA204%2F00%2F0394
n3:rokUplatneniVysledku
n8:2000
n3:svazekPeriodika
73
n3:tvurceVysledku
Svoboda, Augustin Holubářová, Alena Müller, Petr
s:issn
1211-3395
s:numberOfPages
11
n15:organizacniJednotka
14110