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Statements

Subject Item
n2:RIV%2F00216224%3A14110%2F00%3A00003448%21RIV%2F2002%2FGA0%2F141102%2FN
rdf:type
skos:Concept n20:Vysledek
dcterms:description
Four quarternary benzo(c)phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine and two quaternary protoberberine alkaloids berberine and koptisine, were examined for their effects on HeLa cell line (human cervix carcinoma cells) and yeasts S. cerevisiae and Sch. japonicus var. versatilis. The agent concentrations ranged from 1 to 100 mg/ml. Cytotoxicity by neutral red (NR) uptake assay using 96-well plates was tested. In vivo antimicrotubular effect by indirect immunofluorescence of tubulin was tested. ID50 was assessed: sanguinarine ID50 = 0,8 mg/ml, sanguilutine ID50 = 8,3 mg/ml, chelerythrine ID50 = 6,2 mg/ml , chelilutine ID50 = 5,2 mg/ml, coptisine ID50 = 2,6 mg/ml and berberine ID50 =10,0 mg/ml. Alkaloids at lower concentrations (5 to 10 mg/ml) produced a decrease in microtubule numbers, particularly at the cell periphery. The strongest effect was observed in sanguinarine which showed microtubule rearangement at the concentration 0,1 mg/ml and toxic effect Four quarternary benzo(c)phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine and two quaternary protoberberine alkaloids berberine and koptisine, were examined for their effects on HeLa cell line (human cervix carcinoma cells) and yeasts S. cerevisiae and Sch. japonicus var. versatilis. The agent concentrations ranged from 1 to 100 mg/ml. Cytotoxicity by neutral red (NR) uptake assay using 96-well plates was tested. In vivo antimicrotubular effect by indirect immunofluorescence of tubulin was tested. ID50 was assessed: sanguinarine ID50 = 0,8 mg/ml, sanguilutine ID50 = 8,3 mg/ml, chelerythrine ID50 = 6,2 mg/ml , chelilutine ID50 = 5,2 mg/ml, coptisine ID50 = 2,6 mg/ml and berberine ID50 =10,0 mg/ml. Alkaloids at lower concentrations (5 to 10 mg/ml) produced a decrease in microtubule numbers, particularly at the cell periphery. The strongest effect was observed in sanguinarine which showed microtubule rearangement at the concentration 0,1 mg/ml and toxic effect
dcterms:title
The effect of Chelidonius majus alkaloids on tumor and yeast cells Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky
skos:prefLabel
Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky The effect of Chelidonius majus alkaloids on tumor and yeast cells Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky
skos:notation
RIV/00216224:14110/00:00003448!RIV/2002/GA0/141102/N
n3:strany
97
n3:aktivita
n17:P
n3:aktivity
P(GA204/99/D025)
n3:dodaniDat
n10:2002
n3:domaciTvurceVysledku
n6:2115549 n6:3682455 n6:9540539 n6:9382925
n3:druhVysledku
n12:D
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
730932
n3:idVysledku
RIV/00216224:14110/00:00003448
n3:jazykVysledku
n11:cze
n3:klicovaSlova
benzophenenthridine alkaloids, cytotoxicity, cytoskeleton,
n3:klicoveSlovo
n5:benzophenenthridine%20alkaloids n5:cytotoxicity n5:cytoskeleton
n3:kontrolniKodProRIV
[1846AE7025F9]
n3:mistoKonaniAkce
Cěské Budějovice
n3:mistoVydani
Cěské Budějovice
n3:nazevZdroje
Cells II
n3:obor
n15:EA
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n18:GA204%2F99%2FD025
n3:rokUplatneniVysledku
n10:2000
n3:tvurceVysledku
Slaninová, Iva Bochořáková, Hana Táborská, Eva Slanina, Jiří
n3:typAkce
n19:WRD
n3:zahajeniAkce
2000-01-01+01:00
s:numberOfPages
1
n13:hasPublisher
KOPP Publishing
n16:organizacniJednotka
14110