This HTML5 document contains 59 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n17http://localhost/temp/predkladatel/
n13http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216208%3A11320%2F14%3A10287787%21RIV15-MSM-11320___/
n19http://linked.opendata.cz/resource/domain/vavai/projekt/
n10http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n11http://linked.opendata.cz/ontology/domain/vavai/
shttp://schema.org/
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
rdfshttp://www.w3.org/2000/01/rdf-schema#
skoshttp://www.w3.org/2004/02/skos/core#
n18http://bibframe.org/vocab/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n5http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n8http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n20http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n16http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n15http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n7http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216208%3A11320%2F14%3A10287787%21RIV15-MSM-11320___
rdf:type
skos:Concept n11:Vysledek
rdfs:seeAlso
http://nar.oxfordjournals.org/content/42/8/5378.full?sid=eab1a866-369a-4bc8-8838-2585aa47d635
dcterms:description
Several oligothymidylates containing various ratios of phosphodiester and isopolar 5'-hydroxyphosphonate, 5'-O-methylphosphonate and 3'-O-methylphosphonate internucleotide linkages were examined with respect to their hybridization properties with oligoriboadenylates and their ability to induce RNA cleavage by ribonuclease H (RNase H). The results demonstrated that the increasing number of 5'-hydroxyphosphonate or 5'-O-methylphosphonate units in antisense oligonucleotides (AOs) significantly stabilizes the heteroduplexes, whereas 3'-O-methylphosphonate AOs cause strong destabilization of the heteroduplexes. Only the heteroduplexes with 5'-O-methylphosphonate units in the antisense strand exhibited a significant increase in Escherichia coli RNase H cleavage activity by up to 3-fold (depending on the ratio of phosphodiester and phosphonate linkages) in comparison with the natural heteroduplex. A similar increase in RNase H cleavage activity was also observed for heteroduplexes composed of miRNA191 and complementary AOs containing 5'-O-methylphosphonate units. We propose for this type of AOs, working via the RNase H mechanism, the abbreviation MEPNA (MEthylPhosphonate Nucleic Acid). Several oligothymidylates containing various ratios of phosphodiester and isopolar 5'-hydroxyphosphonate, 5'-O-methylphosphonate and 3'-O-methylphosphonate internucleotide linkages were examined with respect to their hybridization properties with oligoriboadenylates and their ability to induce RNA cleavage by ribonuclease H (RNase H). The results demonstrated that the increasing number of 5'-hydroxyphosphonate or 5'-O-methylphosphonate units in antisense oligonucleotides (AOs) significantly stabilizes the heteroduplexes, whereas 3'-O-methylphosphonate AOs cause strong destabilization of the heteroduplexes. Only the heteroduplexes with 5'-O-methylphosphonate units in the antisense strand exhibited a significant increase in Escherichia coli RNase H cleavage activity by up to 3-fold (depending on the ratio of phosphodiester and phosphonate linkages) in comparison with the natural heteroduplex. A similar increase in RNase H cleavage activity was also observed for heteroduplexes composed of miRNA191 and complementary AOs containing 5'-O-methylphosphonate units. We propose for this type of AOs, working via the RNase H mechanism, the abbreviation MEPNA (MEthylPhosphonate Nucleic Acid).
dcterms:title
5 '-O-Methylphosphonate nucleic acids-new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes 5 '-O-Methylphosphonate nucleic acids-new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes
skos:prefLabel
5 '-O-Methylphosphonate nucleic acids-new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes 5 '-O-Methylphosphonate nucleic acids-new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes
skos:notation
RIV/00216208:11320/14:10287787!RIV15-MSM-11320___
n4:aktivita
n20:P n20:I
n4:aktivity
I, P(GA13-26526S), P(GA203/09/0820)
n4:cisloPeriodika
42
n4:dodaniDat
n7:2015
n4:domaciTvurceVysledku
n10:7768486 n10:5110874
n4:druhVysledku
n16:J
n4:duvernostUdaju
n8:S
n4:entitaPredkladatele
n13:predkladatel
n4:idSjednocenehoVysledku
58314
n4:idVysledku
RIV/00216208:11320/14:10287787
n4:jazykVysledku
n14:eng
n4:klicovaSlova
hybrid duplexes; cleavage rate; RNase H; Escherichia coli; modified DNAs; nucleic acids; 5'-O-Methylphosphonate
n4:klicoveSlovo
n5:RNase%20H n5:modified%20DNAs n5:hybrid%20duplexes n5:Escherichia%20coli n5:nucleic%20acids n5:5%27-O-Methylphosphonate n5:cleavage%20rate
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[B93C2FBFF547]
n4:nazevZdroje
Nucleic Acids Research
n4:obor
n15:JA
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
13
n4:projekt
n19:GA13-26526S n19:GA203%2F09%2F0820
n4:rokUplatneniVysledku
n7:2014
n4:svazekPeriodika
2014
n4:tvurceVysledku
Barvík, Ivan Springer, Tomas Simak, Ondrej Petrova, Magdalena Rosenbergova, Sarka Pav, Ondrej Rosenberg, Ivan Novák, Pavel Rejman, Dominik Štěpánek, Josef Liboska, Radek Homola, Jiří Sipova, Hana
n4:wos
000336092300060
s:issn
0305-1048
s:numberOfPages
12
n18:doi
10.1093/nar/gku125
n17:organizacniJednotka
11320