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Statements

Subject Item
n2:RIV%2F00216208%3A11320%2F13%3A10173450%21RIV14-MSM-11320___
rdf:type
n5:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1016/j.jlumin.2011.12.033
dcterms:description
Spectral- and time-resolved infrared phosphorescence set-up was adapted for detection from surfaces of solid samples by utilizing fiber optics. Its abilities are demonstrated on the detection of singlet oxygen photosensitization by intrinsic (protoporphyrin IX synthesized from ALA) and extrinsic (TPPS4 and TMPyP) photosensitizers in in vitro layers of cultured 3T3 murine fibroblasts and HeLa cells mimicking in vivo tissues. Complex decays of phosphorescence of the photosensitizers were detected. The data were approximated by multi-exponential decays, however, no straightforward explanation of the individual components was found. Singlet oxygen phosphorescence kinetics were obtained with rise-times corresponding to singlet oxygen lifetimes ranging from 0.7 mu s to 1.0 mu s and single-exponential decay times between 5.3 mu s and 6.5 mu s for different photosensitizers and cell lines. Spectral- and time-resolved infrared phosphorescence set-up was adapted for detection from surfaces of solid samples by utilizing fiber optics. Its abilities are demonstrated on the detection of singlet oxygen photosensitization by intrinsic (protoporphyrin IX synthesized from ALA) and extrinsic (TPPS4 and TMPyP) photosensitizers in in vitro layers of cultured 3T3 murine fibroblasts and HeLa cells mimicking in vivo tissues. Complex decays of phosphorescence of the photosensitizers were detected. The data were approximated by multi-exponential decays, however, no straightforward explanation of the individual components was found. Singlet oxygen phosphorescence kinetics were obtained with rise-times corresponding to singlet oxygen lifetimes ranging from 0.7 mu s to 1.0 mu s and single-exponential decay times between 5.3 mu s and 6.5 mu s for different photosensitizers and cell lines.
dcterms:title
Spectral- and time-resolved phosphorescence of photosensitizers and singlet oxygen: From in vitro towards in vivo Spectral- and time-resolved phosphorescence of photosensitizers and singlet oxygen: From in vitro towards in vivo
skos:prefLabel
Spectral- and time-resolved phosphorescence of photosensitizers and singlet oxygen: From in vitro towards in vivo Spectral- and time-resolved phosphorescence of photosensitizers and singlet oxygen: From in vitro towards in vivo
skos:notation
RIV/00216208:11320/13:10173450!RIV14-MSM-11320___
n5:predkladatel
n9:orjk%3A11320
n3:aktivita
n18:Z
n3:aktivity
Z(MSM0021620835)
n3:cisloPeriodika
Nov 2013
n3:dodaniDat
n4:2014
n3:domaciTvurceVysledku
n14:2622114 n14:1133268 n14:9749713
n3:druhVysledku
n16:J
n3:duvernostUdaju
n20:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
106975
n3:idVysledku
RIV/00216208:11320/13:10173450
n3:jazykVysledku
n12:eng
n3:klicovaSlova
Cells; PDT; Singlet oxygen; Protoporphyrin IX; TMPyP; TPPS4
n3:klicoveSlovo
n7:Singlet%20oxygen n7:PDT n7:Protoporphyrin%20IX n7:TPPS4 n7:TMPyP n7:Cells
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[54DF58D1A52D]
n3:nazevZdroje
Journal of Luminescence
n3:obor
n21:BO
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:rokUplatneniVysledku
n4:2013
n3:svazekPeriodika
143
n3:tvurceVysledku
Hála, Jan Vyklický, Vojtěch Curkaniuk, Natalija Dědic, Roman
n3:wos
000323912400119
n3:zamer
n19:MSM0021620835
s:issn
0022-2313
s:numberOfPages
5
n6:doi
10.1016/j.jlumin.2011.12.033
n13:organizacniJednotka
11320