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Statements

Subject Item
n2:RIV%2F00216208%3A11320%2F12%3A10129610%21RIV13-GA0-11320___
rdf:type
n11:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1155/2012/674032
dcterms:description
We have introduced a new promising approach for the determination of pKa constants of oligopeptide intrinsic fluorophores and spectral components referring to their differently charged states. The method is based on the factor analysis of multiwavelength spectroscopic pH titration data. As an illustration, we present its application on the study of short segments of the MADS box, which is a highly conserved sequence of a so-called family of transcription factors, by techniques of UV absorption and fluorescence spectroscopies. Investigated oligopeptides contain no tryptophan but one tyrosine serving as an intrinsic fluorophore and absorber. The results indicate both good sensitivity and spectroscopic selectivity of our method, which thus may be considered as a complementary technique to conventional electrochemical methods. We have introduced a new promising approach for the determination of pKa constants of oligopeptide intrinsic fluorophores and spectral components referring to their differently charged states. The method is based on the factor analysis of multiwavelength spectroscopic pH titration data. As an illustration, we present its application on the study of short segments of the MADS box, which is a highly conserved sequence of a so-called family of transcription factors, by techniques of UV absorption and fluorescence spectroscopies. Investigated oligopeptides contain no tryptophan but one tyrosine serving as an intrinsic fluorophore and absorber. The results indicate both good sensitivity and spectroscopic selectivity of our method, which thus may be considered as a complementary technique to conventional electrochemical methods.
dcterms:title
Spectroscopic Determination of pKa Constants of MADS Box Segments Spectroscopic Determination of pKa Constants of MADS Box Segments
skos:prefLabel
Spectroscopic Determination of pKa Constants of MADS Box Segments Spectroscopic Determination of pKa Constants of MADS Box Segments
skos:notation
RIV/00216208:11320/12:10129610!RIV13-GA0-11320___
n11:predkladatel
n12:orjk%3A11320
n3:aktivita
n4:I n4:S n4:P
n3:aktivity
I, P(GA202/09/0193), S
n3:cisloPeriodika
5-6
n3:dodaniDat
n7:2013
n3:domaciTvurceVysledku
n9:1892320 n9:5110874
n3:druhVysledku
n19:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
170371
n3:idVysledku
RIV/00216208:11320/12:10129610
n3:jazykVysledku
n8:eng
n3:klicovaSlova
tyrosine; MADS box; fluorescence spectroscopy; dissociation constant; Factor analysis
n3:klicoveSlovo
n5:Factor%20analysis n5:dissociation%20constant n5:fluorescence%20spectroscopy n5:MADS%20box n5:tyrosine
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[C1E7729514E5]
n3:nazevZdroje
SPECTROSCOPY-AN INTERNATIONAL JOURNAL
n3:obor
n17:BO
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
5
n3:projekt
n20:GA202%2F09%2F0193
n3:rokUplatneniVysledku
n7:2012
n3:svazekPeriodika
27
n3:tvurceVysledku
Profantová, Barbora Zentz, Christian Coic, Yves-Marie Štěpánek, Josef Turpin, Pierre-Yves
n3:wos
000306505500026
s:issn
0712-4813
s:numberOfPages
6
n21:doi
10.1155/2012/674032
n18:organizacniJednotka
11320