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Statements

Subject Item
n2:RIV%2F00216208%3A11320%2F12%3A10126644%21RIV13-GA0-11320___
rdf:type
n3:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1016/j.colsurfa.2012.03.012
dcterms:description
Although surface-enhanced (resonance) Raman scattering SE(R)RS spectroscopy is currently employed as a (bio)analytical technique, designing and optimizing of cheaply and easily available SERS-active substrates are still in progress. In this paper, we tested SERS substrates prepared by immobilization of Au colloidal nanoparticles via aminosilane on glass from the point of view of their spectral sensitivity and reproducibility. This preparation procedure is easy, cheap and provides uniform SERS substrates on a large scale. We measured SE(R)RS spectra of free-base cationic 5,10,15,20-tetrakis(1-methyl-4-pyridyl)porphyrin (TMPyP) as a testing biomolecule using a confocal Raman microspectrometer and two excitation wavelengths (514.5 and 632.8 nm). SERS microspectroscopy had several advantages in comparison to macro-Raman technique: namely the possibility of spectral mapping over the surface, shorter collection times and the absence of strong Raman bands from the glass support. Analytical enhancement factor (AEF) of Au substrates between 10(5) and 10(6) and limits of detection (LOD) of TMPyP similar to 5 x 10(-8) M were determined. Excellent spectral reproducibility of Au substrates (relative standard deviation of signal similar to 15%) was proved. Immobilized Au colloidal nanoparticles can be therefore considered as suitable substrates for SE(R)RS (bio)analytical applications. Although surface-enhanced (resonance) Raman scattering SE(R)RS spectroscopy is currently employed as a (bio)analytical technique, designing and optimizing of cheaply and easily available SERS-active substrates are still in progress. In this paper, we tested SERS substrates prepared by immobilization of Au colloidal nanoparticles via aminosilane on glass from the point of view of their spectral sensitivity and reproducibility. This preparation procedure is easy, cheap and provides uniform SERS substrates on a large scale. We measured SE(R)RS spectra of free-base cationic 5,10,15,20-tetrakis(1-methyl-4-pyridyl)porphyrin (TMPyP) as a testing biomolecule using a confocal Raman microspectrometer and two excitation wavelengths (514.5 and 632.8 nm). SERS microspectroscopy had several advantages in comparison to macro-Raman technique: namely the possibility of spectral mapping over the surface, shorter collection times and the absence of strong Raman bands from the glass support. Analytical enhancement factor (AEF) of Au substrates between 10(5) and 10(6) and limits of detection (LOD) of TMPyP similar to 5 x 10(-8) M were determined. Excellent spectral reproducibility of Au substrates (relative standard deviation of signal similar to 15%) was proved. Immobilized Au colloidal nanoparticles can be therefore considered as suitable substrates for SE(R)RS (bio)analytical applications.
dcterms:title
SE(R)RS microspectroscopy of porphyrins on immobilized Au nanoparticles: Testing spectral sensitivity and reproducibility SE(R)RS microspectroscopy of porphyrins on immobilized Au nanoparticles: Testing spectral sensitivity and reproducibility
skos:prefLabel
SE(R)RS microspectroscopy of porphyrins on immobilized Au nanoparticles: Testing spectral sensitivity and reproducibility SE(R)RS microspectroscopy of porphyrins on immobilized Au nanoparticles: Testing spectral sensitivity and reproducibility
skos:notation
RIV/00216208:11320/12:10126644!RIV13-GA0-11320___
n3:predkladatel
n4:orjk%3A11320
n5:aktivita
n16:I n16:P
n5:aktivity
I, P(7AMB12FR023), P(GBP205/12/G118)
n5:cisloPeriodika
Neuveden
n5:dodaniDat
n10:2013
n5:domaciTvurceVysledku
n15:3343928 n15:8292671
n5:druhVysledku
n19:J
n5:duvernostUdaju
n13:S
n5:entitaPredkladatele
n17:predkladatel
n5:idSjednocenehoVysledku
167552
n5:idVysledku
RIV/00216208:11320/12:10126644
n5:jazykVysledku
n21:eng
n5:klicovaSlova
reproducibility; spectral sensitivity; immobilized nanoparticles; porphyrins; SERRS microspectroscopy
n5:klicoveSlovo
n12:spectral%20sensitivity n12:SERRS%20microspectroscopy n12:reproducibility n12:porphyrins n12:immobilized%20nanoparticles
n5:kodStatuVydavatele
NL - Nizozemsko
n5:kontrolniKodProRIV
[6DC4CCBF055D]
n5:nazevZdroje
Colloids and Surfaces A: Physicochemical and Engineering Aspects
n5:obor
n9:BO
n5:pocetDomacichTvurcuVysledku
2
n5:pocetTvurcuVysledku
3
n5:projekt
n14:7AMB12FR023 n14:GBP205%2F12%2FG118
n5:rokUplatneniVysledku
n10:2012
n5:svazekPeriodika
402
n5:tvurceVysledku
Procházka, Marek Šimáková, Petra Hajdukova-Smidova, Natalia
n5:wos
000304840600003
s:issn
0927-7757
s:numberOfPages
5
n8:doi
10.1016/j.colsurfa.2012.03.012
n20:organizacniJednotka
11320