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Statements

Subject Item
n2:RIV%2F00216208%3A11320%2F11%3A10105570%21RIV12-MSM-11320___
rdf:type
skos:Concept n20:Vysledek
rdfs:seeAlso
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038649/?tool=pubmed
dcterms:description
The U4/U6.U5 tri-small nuclear ribonucleoprotein particle (tri-snRNP) is an essential pre-mRNA splicing factor, which is assembled in a stepwise manner before each round of splicing. It was previously shown that the tri-snRNP is formed in Cajal bodies (CBs), but little is known about the dynamics of this process. Here we created a mathematical model of tri-snRNP assembly in CBs and used it to fit kinetics of individual snRNPs monitored by fluorescence recovery after photobleaching. A global fitting of all kinetic data determined key reaction constants of tri-snRNP assembly. Our model predicts that the rates of di-snRNP and tri-snRNP assemblies are similar and that approximately 230 tri-snRNPs are assembled in one CB per minute. Our analysis further indicates that tri-snRNP assembly is approximately 10-fold faster in CBs than in the surrounding nucleoplasm, which is fully consistent with the importance of CBs for snRNP formation in rapidly developing biological systems. Finally, the model predicted binding between SART3 and a CB component. We tested this prediction by Forster resonance energy transfer and revealed an interaction between SART3 and coilin in CBs. The U4/U6.U5 tri-small nuclear ribonucleoprotein particle (tri-snRNP) is an essential pre-mRNA splicing factor, which is assembled in a stepwise manner before each round of splicing. It was previously shown that the tri-snRNP is formed in Cajal bodies (CBs), but little is known about the dynamics of this process. Here we created a mathematical model of tri-snRNP assembly in CBs and used it to fit kinetics of individual snRNPs monitored by fluorescence recovery after photobleaching. A global fitting of all kinetic data determined key reaction constants of tri-snRNP assembly. Our model predicts that the rates of di-snRNP and tri-snRNP assemblies are similar and that approximately 230 tri-snRNPs are assembled in one CB per minute. Our analysis further indicates that tri-snRNP assembly is approximately 10-fold faster in CBs than in the surrounding nucleoplasm, which is fully consistent with the importance of CBs for snRNP formation in rapidly developing biological systems. Finally, the model predicted binding between SART3 and a CB component. We tested this prediction by Forster resonance energy transfer and revealed an interaction between SART3 and coilin in CBs.
dcterms:title
In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies
skos:prefLabel
In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies
skos:notation
RIV/00216208:11320/11:10105570!RIV12-MSM-11320___
n20:predkladatel
n21:orjk%3A11320
n3:aktivita
n6:S n6:P n6:Z
n3:aktivity
P(GA204/07/0133), P(KAN200520801), S, Z(AV0Z50390512), Z(AV0Z50390703), Z(AV0Z50520514), Z(MSM0021620835)
n3:cisloPeriodika
4
n3:dodaniDat
n10:2012
n3:domaciTvurceVysledku
n8:7766017 n8:7948395
n3:druhVysledku
n5:J
n3:duvernostUdaju
n22:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
204058
n3:idVysledku
RIV/00216208:11320/11:10105570
n3:jazykVysledku
n11:eng
n3:klicovaSlova
model; FRET; FRAP; fluorescence; Cajal bodies; formation; tri-snRNP; U4/U6.U5; kinetics
n3:klicoveSlovo
n7:FRET n7:Cajal%20bodies n7:U4%2FU6.U5 n7:formation n7:tri-snRNP n7:kinetics n7:FRAP n7:model n7:fluorescence
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[FF0107B69767]
n3:nazevZdroje
Molecular Biology of the Cell
n3:obor
n16:CE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
5
n3:projekt
n19:KAN200520801 n19:GA204%2F07%2F0133
n3:rokUplatneniVysledku
n10:2011
n3:svazekPeriodika
22
n3:tvurceVysledku
Blažíková, Michaela Staněk, David Novotný, Ivan Malínský, Jan Heřman, Petr
n3:wos
000287305700008
n3:zamer
n9:AV0Z50390703 n9:AV0Z50390512 n9:MSM0021620835 n9:AV0Z50520514
s:issn
1059-1524
s:numberOfPages
11
n14:doi
10.1091/mbc.E10-07-0560
n13:organizacniJednotka
11320