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Statements

Subject Item
n2:RIV%2F00216208%3A11320%2F11%3A10081657%21RIV12-MSM-11320___
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Fluorescence microimaging and homodyne phase-resolved confocal microspectrofluorimetry were used to monitor the transport of antisense oligonucleotide into 3T3 living cells and its subsequent intracellular distribution. Phosphorothioate analog of 15-mer oligothymidylate labeled by ATTO 425 was complexed with 5,10,15,20-tetrakis (1-methyl-4-pyridyl) porphyrin (H2TMPyP4) as an uptake-mediating agent. High frequency (up to 180 MHz) analog modulation of both exciting diode laser and the detector image intensifier gain was used to record time-resolved fluorescence spectra. Fluorescence lifetime data within a broad spectral range have revealed preservation of oligonucleotide/porphyrin complex integrity and binding properties of both components inside the cell. Fluorescence microimaging and homodyne phase-resolved confocal microspectrofluorimetry were used to monitor the transport of antisense oligonucleotide into 3T3 living cells and its subsequent intracellular distribution. Phosphorothioate analog of 15-mer oligothymidylate labeled by ATTO 425 was complexed with 5,10,15,20-tetrakis (1-methyl-4-pyridyl) porphyrin (H2TMPyP4) as an uptake-mediating agent. High frequency (up to 180 MHz) analog modulation of both exciting diode laser and the detector image intensifier gain was used to record time-resolved fluorescence spectra. Fluorescence lifetime data within a broad spectral range have revealed preservation of oligonucleotide/porphyrin complex integrity and binding properties of both components inside the cell.
dcterms:title
Cellular uptake of modified oligonucleotides enhanced by porphyrins studied by time-resolved microspectrofluorimetry and fluorescence imaging techniques Cellular uptake of modified oligonucleotides enhanced by porphyrins studied by time-resolved microspectrofluorimetry and fluorescence imaging techniques
skos:prefLabel
Cellular uptake of modified oligonucleotides enhanced by porphyrins studied by time-resolved microspectrofluorimetry and fluorescence imaging techniques Cellular uptake of modified oligonucleotides enhanced by porphyrins studied by time-resolved microspectrofluorimetry and fluorescence imaging techniques
skos:notation
RIV/00216208:11320/11:10081657!RIV12-MSM-11320___
n14:predkladatel
n16:orjk%3A11320
n3:aktivita
n19:Z
n3:aktivity
Z(MSM0021620835)
n3:cisloPeriodika
1-3
n3:dodaniDat
n12:2012
n3:domaciTvurceVysledku
n4:2219387 n4:5110874 n4:9873856 n4:2504634
n3:druhVysledku
n15:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n5:predkladatel
n3:idSjednocenehoVysledku
189389
n3:idVysledku
RIV/00216208:11320/11:10081657
n3:jazykVysledku
n6:eng
n3:klicovaSlova
fluorescence imaging; time-resolved microspectrofluorimetry; fluorescence lifetime; porphyrin
n3:klicoveSlovo
n10:fluorescence%20imaging n10:time-resolved%20microspectrofluorimetry n10:fluorescence%20lifetime n10:porphyrin
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[E455048D9F9B]
n3:nazevZdroje
Journal of Molecular Structure
n3:obor
n18:BO
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n12:2011
n3:svazekPeriodika
993
n3:tvurceVysledku
Mojzeš, Peter Praus, Petr Turpin, Pierre-Yves Kočišová, Eva Štěpánek, Josef Sureau, Franck
n3:wos
000291066000050
n3:zamer
n9:MSM0021620835
s:issn
0022-2860
s:numberOfPages
3
n11:doi
10.1016/j.molstruc.2011.01.061
n20:organizacniJednotka
11320