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Statements

Subject Item
n2:RIV%2F00216208%3A11320%2F10%3A10072373%21RIV11-GA0-11320___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Raman titration experiment with magnesium salt added gradually to aqueous solution of duplexes formed by RNA homopolynucleotides polyU and polyA was performed to reveal its effect on homopolynucleotide complexes. Statistical analysis of obtained spectral set has confirmed the effect already found by less structurally sensitive methods [Nucleic Acids Res. 31(17) (2003), 5101-5107] that at sufficiently high concentrations magnesium causes transformation of polyU-polyA duplexes to polyU-polyA-polyU triplexes and single polyA strands. It was found that at relatively high polynucleotide concentrations used in Raman experiment, the threshold magnesium concentration for this effect is above the concentration of duplex base-pairs in solution. Due to the strong spectral changes attributed to the varied percentages of duplexes, triplexes and single strands, it was not possible to register weaker direct Raman signs of the magnesium binding to polynucleotide strand. Raman titration experiment with magnesium salt added gradually to aqueous solution of duplexes formed by RNA homopolynucleotides polyU and polyA was performed to reveal its effect on homopolynucleotide complexes. Statistical analysis of obtained spectral set has confirmed the effect already found by less structurally sensitive methods [Nucleic Acids Res. 31(17) (2003), 5101-5107] that at sufficiently high concentrations magnesium causes transformation of polyU-polyA duplexes to polyU-polyA-polyU triplexes and single polyA strands. It was found that at relatively high polynucleotide concentrations used in Raman experiment, the threshold magnesium concentration for this effect is above the concentration of duplex base-pairs in solution. Due to the strong spectral changes attributed to the varied percentages of duplexes, triplexes and single strands, it was not possible to register weaker direct Raman signs of the magnesium binding to polynucleotide strand.
dcterms:title
Raman study of magnesium induced conversion of polyU-polyA-polyU triplexes Raman study of magnesium induced conversion of polyU-polyA-polyU triplexes
skos:prefLabel
Raman study of magnesium induced conversion of polyU-polyA-polyU triplexes Raman study of magnesium induced conversion of polyU-polyA-polyU triplexes
skos:notation
RIV/00216208:11320/10:10072373!RIV11-GA0-11320___
n3:aktivita
n9:S n9:P n9:Z
n3:aktivity
P(GA202/09/0193), P(GD202/09/H041), S, Z(MSM0021620835)
n3:cisloPeriodika
3-4
n3:dodaniDat
n11:2011
n3:domaciTvurceVysledku
n10:6249701 n10:5110874
n3:druhVysledku
n18:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
283855
n3:idVysledku
RIV/00216208:11320/10:10072373
n3:jazykVysledku
n19:eng
n3:klicovaSlova
polyA; polyU; triplex; duplex; magnesium; Raman
n3:klicoveSlovo
n5:polyU n5:polyA n5:magnesium n5:Raman n5:duplex n5:triplex
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[7B5E5948850E]
n3:nazevZdroje
SPECTROSCOPY-AN INTERNATIONAL JOURNAL
n3:obor
n15:BO
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n4:GD202%2F09%2FH041 n4:GA202%2F09%2F0193
n3:rokUplatneniVysledku
n11:2010
n3:svazekPeriodika
24
n3:tvurceVysledku
Štěpánek, Josef Espinoza Herrera, Shirly Josefina
n3:wos
000281667400041
n3:zamer
n8:MSM0021620835
s:issn
0712-4813
s:numberOfPages
4
n17:organizacniJednotka
11320