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Statements

Subject Item
n2:RIV%2F00216208%3A11320%2F10%3A10070564%21RIV11-MSM-11320___
rdf:type
skos:Concept n11:Vysledek
dcterms:description
Time-resolved confocal microspectrofluorometry and fluorescence microimaging were used to monitor how the model antisense oligonucleotide is transported into 3T3 living cells and distributed inside them. Phosphorothioate analog of 15-mer oligothymidylate labeled by ATTO 425 was complexed with Zn(II) 5,10,15,20-tetrakis(4-N-methylpyridyl) porphyrin as an uptake-mediating agent. Homodyne phase-resolved technique based on a high frequency analog modulation of both exciting diode laser and detector image intensifier was used for time-resolved measurements. Decay-time data obtained within a broad range spectral region have provided unique information about the fate of both fluorophores inside the cell. Time-resolved confocal microspectrofluorometry and fluorescence microimaging were used to monitor how the model antisense oligonucleotide is transported into 3T3 living cells and distributed inside them. Phosphorothioate analog of 15-mer oligothymidylate labeled by ATTO 425 was complexed with Zn(II) 5,10,15,20-tetrakis(4-N-methylpyridyl) porphyrin as an uptake-mediating agent. Homodyne phase-resolved technique based on a high frequency analog modulation of both exciting diode laser and detector image intensifier was used for time-resolved measurements. Decay-time data obtained within a broad range spectral region have provided unique information about the fate of both fluorophores inside the cell.
dcterms:title
Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution
skos:prefLabel
Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution
skos:notation
RIV/00216208:11320/10:10070564!RIV11-MSM-11320___
n3:aktivita
n18:Z
n3:aktivity
Z(MSM0021620835)
n3:cisloPeriodika
3-4
n3:dodaniDat
n15:2011
n3:domaciTvurceVysledku
n4:9873856 n4:5110874 n4:2504634 n4:2219387
n3:druhVysledku
n13:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
259806
n3:idVysledku
RIV/00216208:11320/10:10070564
n3:jazykVysledku
n7:eng
n3:klicovaSlova
cellular uptake; microspectrofluorometry; porphyrin; oligonucleotide; Fluorescence lifetime
n3:klicoveSlovo
n12:oligonucleotide n12:cellular%20uptake n12:microspectrofluorometry n12:porphyrin n12:Fluorescence%20lifetime
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[51206013413F]
n3:nazevZdroje
SPECTROSCOPY-AN INTERNATIONAL JOURNAL
n3:obor
n5:BO
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n15:2010
n3:svazekPeriodika
24
n3:tvurceVysledku
Praus, Petr Štěpánek, Josef Mojzeš, Peter Turpin, Pierre-Yves Kočišová, Eva Sureau, Franck
n3:wos
000281667400019
n3:zamer
n14:MSM0021620835
s:issn
0712-4813
s:numberOfPages
5
n10:organizacniJednotka
11320