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Subject Item
n2:RIV%2F00216208%3A11310%2F14%3A10293151%21RIV15-MSM-11310___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
This chapter introduces plant cell strains and describes their main characteristics which render them unique experimental models to study plant morphogenesis. Although composed only of few cells, these systems are characterised by strict natural polarity of cell growth and division and high spontaneous friability of the daughter cell files. Both features are most clearly exhibited in two tobacco cell lines, Virginia Bright Italia (VBI-0) and Bright Yellow (BY-2). Owing to their ability to grow in vitro resembling animal single cell layer systems, they offer the possibility to accompany molecular or biochemical analyses of the material by a precise but nondestructive in vivo cytological observation of the cell population. Here we summarise the use of these two lines in various plant phenotyping studies including investigations of morphoregulatory signals like phytohormones or stress factors as well as organisation of cytoskeleton, endomembranes and cell wall. Although so far not frequently utilised in practical applications, some transgenic BY-2 strains show high potential for molecular farming. Last but not the least, the mechanisms responsible for the spontaneous or targeted selection of similar plant cell strains from genetically more attractive plant species are discussed. This chapter introduces plant cell strains and describes their main characteristics which render them unique experimental models to study plant morphogenesis. Although composed only of few cells, these systems are characterised by strict natural polarity of cell growth and division and high spontaneous friability of the daughter cell files. Both features are most clearly exhibited in two tobacco cell lines, Virginia Bright Italia (VBI-0) and Bright Yellow (BY-2). Owing to their ability to grow in vitro resembling animal single cell layer systems, they offer the possibility to accompany molecular or biochemical analyses of the material by a precise but nondestructive in vivo cytological observation of the cell population. Here we summarise the use of these two lines in various plant phenotyping studies including investigations of morphoregulatory signals like phytohormones or stress factors as well as organisation of cytoskeleton, endomembranes and cell wall. Although so far not frequently utilised in practical applications, some transgenic BY-2 strains show high potential for molecular farming. Last but not the least, the mechanisms responsible for the spontaneous or targeted selection of similar plant cell strains from genetically more attractive plant species are discussed.
dcterms:title
Plant cell strains in fundamental research and applications. Plant cell strains in fundamental research and applications.
skos:prefLabel
Plant cell strains in fundamental research and applications. Plant cell strains in fundamental research and applications.
skos:notation
RIV/00216208:11310/14:10293151!RIV15-MSM-11310___
n3:aktivita
n12:I
n3:aktivity
I
n3:dodaniDat
n10:2015
n3:domaciTvurceVysledku
n4:6601626 n4:5408954
n3:druhVysledku
n8:C
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
36752
n3:idVysledku
RIV/00216208:11310/14:10293151
n3:jazykVysledku
n11:eng
n3:klicovaSlova
applications.; research; fundamental; strains; cell; Plant
n3:klicoveSlovo
n7:cell n7:Plant n7:research n7:fundamental n7:strains n7:applications.
n3:kontrolniKodProRIV
[C8418E33DD59]
n3:mistoVydani
neuveden
n3:nazevEdiceCisloSvazku
Plant Cell Monographs, 22
n3:nazevZdroje
Plant Cell Strains in Fundamental Research and Applications
n3:obor
n13:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetStranKnihy
481
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n10:2014
n3:tvurceVysledku
Opatrný, Zdeněk Petrášek, Jan Nick, Peter
s:numberOfPages
26
n14:doi
10.1007/978-3-642-41787-0_15
n18:hasPublisher
Springer-Verlag. (Berlin; Heidelberg)
n20:isbn
978-3-642-41786-3
n9:organizacniJednotka
11310