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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F14%3A10290813%21RIV15-MSM-11310___
rdf:type
n5:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1017/S0007485314000224
dcterms:description
Several species of the genus Cryptolestes Ganglbauer, 1899 (Coleoptera: Laemophloeidae) are commonly found in stored products. In this study, five species of Cryptolestes, with almost worldwide distribution, were obtained from laboratories in China, Czech Republic and the USA: Cryptolestes ferrugineus (Stephens, 1831), Cryptolestes pusillus (Schonherr, 1817), Cryptolestes turcicus (Grouvelle, 1876), Cryptolestes pusilloides (Steel & Howe, 1952) and Cryptolestes capensis (Waltl, 1834). Molecular identification based on a 658bp fragment from the mitochondrial DNA cytochrome c oxidase subunit I (COI) was adopted to overcome some problems of morphological identification of Cryptolestes species. The utility of COI sequences as DNA barcodes in discriminating the five Cryptolestes species was evaluated on adults and larvae by analysing Kimura 2-parameter distances, phylogenetic tree and haplotype networks. The results showed that molecular approaches based on DNA barcodes were able to accurately identify these species. This is the first study using DNA barcoding to identify Cryptolestes species and the gathered DNA sequences will complement the biological barcode database. Several species of the genus Cryptolestes Ganglbauer, 1899 (Coleoptera: Laemophloeidae) are commonly found in stored products. In this study, five species of Cryptolestes, with almost worldwide distribution, were obtained from laboratories in China, Czech Republic and the USA: Cryptolestes ferrugineus (Stephens, 1831), Cryptolestes pusillus (Schonherr, 1817), Cryptolestes turcicus (Grouvelle, 1876), Cryptolestes pusilloides (Steel & Howe, 1952) and Cryptolestes capensis (Waltl, 1834). Molecular identification based on a 658bp fragment from the mitochondrial DNA cytochrome c oxidase subunit I (COI) was adopted to overcome some problems of morphological identification of Cryptolestes species. The utility of COI sequences as DNA barcodes in discriminating the five Cryptolestes species was evaluated on adults and larvae by analysing Kimura 2-parameter distances, phylogenetic tree and haplotype networks. The results showed that molecular approaches based on DNA barcodes were able to accurately identify these species. This is the first study using DNA barcoding to identify Cryptolestes species and the gathered DNA sequences will complement the biological barcode database.
dcterms:title
DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae) DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae)
skos:prefLabel
DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae) DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae)
skos:notation
RIV/00216208:11310/14:10290813!RIV15-MSM-11310___
n4:aktivita
n7:P n7:I
n4:aktivity
I, P(LH12160)
n4:cisloPeriodika
5
n4:dodaniDat
n15:2015
n4:domaciTvurceVysledku
n14:4123549
n4:druhVysledku
n12:J
n4:duvernostUdaju
n19:S
n4:entitaPredkladatele
n18:predkladatel
n4:idSjednocenehoVysledku
11991
n4:idVysledku
RIV/00216208:11310/14:10290813
n4:jazykVysledku
n11:eng
n4:klicovaSlova
COI; DNA barcoding; identification; stored-product pests; Cryptolestes
n4:klicoveSlovo
n10:DNA%20barcoding n10:stored-product%20pests n10:Cryptolestes n10:identification n10:COI
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[148694EE01A8]
n4:nazevZdroje
Bulletin of Entomological Research
n4:obor
n8:GF
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
10
n4:projekt
n16:LH12160
n4:rokUplatneniVysledku
n15:2014
n4:svazekPeriodika
104
n4:tvurceVysledku
Li, F. J. Zhang, S. F. Cao, Y. Li, Z. H. Wang, Y. J. Varadínová, Zuzana Kučerová, Z. Jiang, F. Stejskal, V. Opit, G.
n4:wos
000342200200014
s:issn
0007-4853
s:numberOfPages
8
n13:doi
10.1017/S0007485314000224
n17:organizacniJednotka
11310