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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F14%3A10287961%21RIV15-MSM-11310___
rdf:type
n7:Vysledek skos:Concept
dcterms:description
A low-molecular-weight (under 10 kDa) dialysable leukocyte extract (called transfer factor, TF) has been shown to be a prospective substance to improve or modulate immune response in autoimmunity, inflammation, infectious diseases or cancers. However, the use of TF has been limited by the absence of any data on the mechanism of its action. Here we show that TF prepared from peripheral blood leukocytes of healthy human donors displays multiple regulatory effects on individual parameters of the immune system. TF decreases proliferation of T and B lymphocytes and partially alters the production of cytokines and nitric oxide by activated macrophages. TF also inhibits production of T helper 1 (Th1) cytokines interleukin 2 (IL-2) and interferon γ, slightly stimulates production of Th2 cytokine IL-10 and considerably enhances the secretion of IL-17 by activated mouse spleen T cells. At the molecular level, TF enhances expression of genes for transcription factor RORγt and for IL-17. The enhanced expression of the RORgt gene corresponds with an increase in the number of RORγt+CD4+ Th17 cells and with enhanced IL-17 production. In contrast, the expression of the Foxp3 gene and the proportion of CD4+CD25+Foxp3+ regulatory T cells are not significantly changed in the presence of TF. These results suggest that the activation of pro-inflammatory Th17 cells, which have multiple immunoregulatory properties, could be the main mechanism of the immunomodulatory action of a low-molecular-weight leukocyte extract. A low-molecular-weight (under 10 kDa) dialysable leukocyte extract (called transfer factor, TF) has been shown to be a prospective substance to improve or modulate immune response in autoimmunity, inflammation, infectious diseases or cancers. However, the use of TF has been limited by the absence of any data on the mechanism of its action. Here we show that TF prepared from peripheral blood leukocytes of healthy human donors displays multiple regulatory effects on individual parameters of the immune system. TF decreases proliferation of T and B lymphocytes and partially alters the production of cytokines and nitric oxide by activated macrophages. TF also inhibits production of T helper 1 (Th1) cytokines interleukin 2 (IL-2) and interferon γ, slightly stimulates production of Th2 cytokine IL-10 and considerably enhances the secretion of IL-17 by activated mouse spleen T cells. At the molecular level, TF enhances expression of genes for transcription factor RORγt and for IL-17. The enhanced expression of the RORgt gene corresponds with an increase in the number of RORγt+CD4+ Th17 cells and with enhanced IL-17 production. In contrast, the expression of the Foxp3 gene and the proportion of CD4+CD25+Foxp3+ regulatory T cells are not significantly changed in the presence of TF. These results suggest that the activation of pro-inflammatory Th17 cells, which have multiple immunoregulatory properties, could be the main mechanism of the immunomodulatory action of a low-molecular-weight leukocyte extract.
dcterms:title
A Low-Molecular-Weight Dialysable Leukocyte Extract Selectively Enhances Development of CD4+RORγt+ T Cells and IL-17 Production A Low-Molecular-Weight Dialysable Leukocyte Extract Selectively Enhances Development of CD4+RORγt+ T Cells and IL-17 Production
skos:prefLabel
A Low-Molecular-Weight Dialysable Leukocyte Extract Selectively Enhances Development of CD4+RORγt+ T Cells and IL-17 Production A Low-Molecular-Weight Dialysable Leukocyte Extract Selectively Enhances Development of CD4+RORγt+ T Cells and IL-17 Production
skos:notation
RIV/00216208:11310/14:10287961!RIV15-MSM-11310___
n3:aktivita
n16:I
n3:aktivity
I
n3:cisloPeriodika
6
n3:dodaniDat
n9:2015
n3:domaciTvurceVysledku
n4:9565612 n4:3650871 n4:5695678 n4:7357354 n4:2767198
n3:druhVysledku
n14:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
804
n3:idVysledku
RIV/00216208:11310/14:10287961
n3:jazykVysledku
n10:eng
n3:klicovaSlova
Th17 cells; RORgt; low-molecular weight leukocyte extract; IL-17; cytokine production
n3:klicoveSlovo
n6:Th17%20cells n6:RORgt n6:cytokine%20production n6:IL-17 n6:low-molecular%20weight%20leukocyte%20extract
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[FEDC5AE0472A]
n3:nazevZdroje
Folia Biologica
n3:obor
n5:EC
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n9:2014
n3:svazekPeriodika
60
n3:tvurceVysledku
Holáň, Vladimír Zajícová, Alena Krulová, Magdaléna Chudíčková, Milada Javorková, Eliška Trošan, Peter
n3:wos
000347748000001
s:issn
0015-5500
s:numberOfPages
8
n12:organizacniJednotka
11310