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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F14%3A10287808%21RIV15-MSM-11310___
rdf:type
n12:Vysledek skos:Concept
dcterms:description
The efficacy of a chemically modified dextran - heparan sulfate mimicking regenerating agent (RGTA) on the healing of the rabbit cornea injured with alkali was examined. The eyes were injured with 0.15 N NaOH applied on the cornea or with 1.0 N NaOH using a 8 mm diameter filter paper disk. Then RGTA or placebo was applied on the cornea. In the last group of rabbits, corneas injured with the high alkali concentration were left without any treatment for four weeks; subsequently, the corneas were treated with RGTA or placebo. The central corneal thickness was measured using a pachymeter. The corneas were examined morphologically, immunohistochemically and for real time-PCR. Compared to control (unaffected) corneas, following the application of low alkali concentration the expression of urokinase-type plasminogen activator, metalloproteinase 9, nitric oxide synthase and xanthine oxidase was increased in the injured corneal epithelium of placebo-treated eyes, whereas the expression of antioxidant enzymes was reduced. Nitrotyrosine and malondialdehyde stainings appeared in the corneal epithelium. RGTA application suppressed the antioxidant/prooxidant imbalance and reduced the expression of the above-mentioned immunohistochemical markers. The corneal thickness increased after alkali injury, decreased during corneal healing after RGTA treatment faster than after placebo application. Following the injury with the high alkali concentration, corneal inflammation and neovascularization were highly pronounced in placebo-treated corneas, whereas in RGTA-treated corneas they were significantly supressed. When RGTA or placebo application was started later after alkali injury and corneas were ulcerated, subsequent RGTA treatment healed the majority of them. In conclusion, RGTA facilitates the healing of injured corneas via a reduction of proteolytic, oxidative and nitrosative damage. The efficacy of a chemically modified dextran - heparan sulfate mimicking regenerating agent (RGTA) on the healing of the rabbit cornea injured with alkali was examined. The eyes were injured with 0.15 N NaOH applied on the cornea or with 1.0 N NaOH using a 8 mm diameter filter paper disk. Then RGTA or placebo was applied on the cornea. In the last group of rabbits, corneas injured with the high alkali concentration were left without any treatment for four weeks; subsequently, the corneas were treated with RGTA or placebo. The central corneal thickness was measured using a pachymeter. The corneas were examined morphologically, immunohistochemically and for real time-PCR. Compared to control (unaffected) corneas, following the application of low alkali concentration the expression of urokinase-type plasminogen activator, metalloproteinase 9, nitric oxide synthase and xanthine oxidase was increased in the injured corneal epithelium of placebo-treated eyes, whereas the expression of antioxidant enzymes was reduced. Nitrotyrosine and malondialdehyde stainings appeared in the corneal epithelium. RGTA application suppressed the antioxidant/prooxidant imbalance and reduced the expression of the above-mentioned immunohistochemical markers. The corneal thickness increased after alkali injury, decreased during corneal healing after RGTA treatment faster than after placebo application. Following the injury with the high alkali concentration, corneal inflammation and neovascularization were highly pronounced in placebo-treated corneas, whereas in RGTA-treated corneas they were significantly supressed. When RGTA or placebo application was started later after alkali injury and corneas were ulcerated, subsequent RGTA treatment healed the majority of them. In conclusion, RGTA facilitates the healing of injured corneas via a reduction of proteolytic, oxidative and nitrosative damage.
dcterms:title
The healing of alkali-injured cornea is stimulated by a novel matrix regenerating agent (RGTA, CACICOL20) - a biopolymer mimicking heparan sulfates reducing proteolytic, oxidative and nitrosative damage The healing of alkali-injured cornea is stimulated by a novel matrix regenerating agent (RGTA, CACICOL20) - a biopolymer mimicking heparan sulfates reducing proteolytic, oxidative and nitrosative damage
skos:prefLabel
The healing of alkali-injured cornea is stimulated by a novel matrix regenerating agent (RGTA, CACICOL20) - a biopolymer mimicking heparan sulfates reducing proteolytic, oxidative and nitrosative damage The healing of alkali-injured cornea is stimulated by a novel matrix regenerating agent (RGTA, CACICOL20) - a biopolymer mimicking heparan sulfates reducing proteolytic, oxidative and nitrosative damage
skos:notation
RIV/00216208:11310/14:10287808!RIV15-MSM-11310___
n3:aktivita
n4:P n4:Z n4:I
n3:aktivity
I, P(GAP304/11/0653), Z(AV0Z50390512)
n3:cisloPeriodika
4
n3:dodaniDat
n11:2015
n3:domaciTvurceVysledku
n9:7357354 n9:5695678
n3:druhVysledku
n18:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
18829
n3:idVysledku
RIV/00216208:11310/14:10287808
n3:jazykVysledku
n8:eng
n3:klicovaSlova
RGTA treatment; Alkali-injured cornea
n3:klicoveSlovo
n6:Alkali-injured%20cornea n6:RGTA%20treatment
n3:kodStatuVydavatele
ES - Španělské království
n3:kontrolniKodProRIV
[BFA0D2F9C015]
n3:nazevZdroje
Histology and Histopathology
n3:obor
n19:EC
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
5
n3:projekt
n17:GAP304%2F11%2F0653
n3:rokUplatneniVysledku
n11:2014
n3:svazekPeriodika
29
n3:tvurceVysledku
Holáň, Vladimír Trošan, Peter Čejková, Jitka Olmiere, Céline Čejka, Čestmír
n3:wos
000333756800006
n3:zamer
n14:AV0Z50390512
s:issn
0213-3911
s:numberOfPages
22
n13:organizacniJednotka
11310