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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F14%3A10285804%21RIV15-MSM-11310___
rdf:type
skos:Concept n14:Vysledek
rdfs:seeAlso
http://www.bestservis.eu/upload/file/Sbornik_metody14.pdf
dcterms:description
Elektrochemický enzymatický reaktor s práškovým amalgámem byl navržen jako hlavní část biosenzoru pro průtokové systémy. Enzymatic reactor based on the powder of silver solid amalgam was suggested as the main part of biosensors in flow systems for the first time. 4-aminothiophenol, glutaraldehyde and enzyme were gradually bonded to the amalgam surface. Large surface of the fine amalgam particles maintains a big quantity of enzyme molecules. Biosensors were tested with enzymes ascorbate oxidase, glucose oxidase, catalase, tyrosinase and laccase. Electrochemical detection of the oxygen concentration change (for the first 3 enzymes) or the quinones concentration (tyrosinase and laccase) in the measured solution was done amperometrically by means of the tubular detector of silver solid amalgam. The current response of each biosensor was optimized with respect to the detection potential, the flow rate of the mobile phase, the injection volume and the enzymatic reactor volume. Under the found optimum conditions, concentration dependence and some statistical parameters of repeated measurements (relative standard deviation (RSD) for the studied enzymes was in the range 0.81 - 2.1 %) were measured. Biosensor with the ascorbate oxidase reactor was used for determination of ascorbic acid in the vitamin tablet Celaskon(R). Results of the analysis were in good agreement with the contents of ascorbic acid declared by manufacturer and the RSD of these analyses was 2.0 %. Elektrochemický enzymatický reaktor s práškovým amalgámem byl navržen jako hlavní část biosenzoru pro průtokové systémy.
dcterms:title
Elektrochemické enzymatické biosenzory na základě reaktoru s práškovým amalgámem Elektrochemické enzymatické biosenzory na základě reaktoru s práškovým amalgámem Electrochemical Biosensors Based on Enzymatic Reactor with Amalgam Powder
skos:prefLabel
Elektrochemické enzymatické biosenzory na základě reaktoru s práškovým amalgámem Electrochemical Biosensors Based on Enzymatic Reactor with Amalgam Powder Elektrochemické enzymatické biosenzory na základě reaktoru s práškovým amalgámem
skos:notation
RIV/00216208:11310/14:10285804!RIV15-MSM-11310___
n6:aktivita
n19:S n19:I
n6:aktivity
I, S
n6:dodaniDat
n10:2015
n6:domaciTvurceVysledku
n15:1839594 n15:4454472
n6:druhVysledku
n13:D
n6:duvernostUdaju
n21:S
n6:entitaPredkladatele
n7:predkladatel
n6:idSjednocenehoVysledku
14247
n6:idVysledku
RIV/00216208:11310/14:10285804
n6:jazykVysledku
n11:cze
n6:klicovaSlova
Powdered enzymatic reactor; Solid amalgam; Amperometry; Flow analysis; Electrochemical biosensors
n6:klicoveSlovo
n9:Flow%20analysis n9:Powdered%20enzymatic%20reactor n9:Solid%20amalgam n9:Electrochemical%20biosensors n9:Amperometry
n6:kontrolniKodProRIV
[334CD7892ED1]
n6:mistoKonaniAkce
Jetřichovice, Czech Republic
n6:mistoVydani
Ústí nad Labem
n6:nazevZdroje
Sborník přednášek mezinárodní odborné konference XXXIV. Moderní Elektrochemické Metody Jetřichovice 19.- 23. května 2014
n6:obor
n12:CG
n6:pocetDomacichTvurcuVysledku
2
n6:pocetTvurcuVysledku
3
n6:rokUplatneniVysledku
n10:2014
n6:tvurceVysledku
Barek, Jiří Josypčuk, Oksana Josypčuk, Bohdan
n6:typAkce
n17:EUR
n6:zahajeniAkce
2014-05-19+02:00
s:numberOfPages
5
n18:hasPublisher
Srsenová Lenka - Best servis Ústí nad Labem
n16:isbn
978-80-905221-2-1
n20:organizacniJednotka
11310