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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F14%3A10283468%21RIV15-MSM-11310___
rdf:type
n14:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1371/journal.pone.0086247
dcterms:description
It is now clear that whole genome duplications have occurred in all eukaryotic evolutionary lineages, and that the vast majority of flowering plants have experienced polyploidisation in their evolutionary history. However, study of genome size variation in microalgae lags behind that of higher plants and seaweeds. In this study, we have addressed the question whether microalgal phylogeny is associated with DNA content variation in order to evaluate the evolutionary significance of polyploidy in the model genus Micrasterias. We applied flow-cytometric techniques of DNA quantification to microalgae and mapped the estimated DNA content along the phylogenetic tree. Correlations between DNA content and cell morphometric parameters were also tested using geometric morphometrics. In total, DNA content was successfully determined for 34 strains of the genus Micrasterias. The estimated absolute 2C nuclear DNA amount ranged from 2.1 to 64.7 pg; intraspecific variation being 17.4-30.7 pg in M. truncata and 32.0-64.7 pg in M. rotata. There were significant differences between DNA contents of related species. We found strong correlation between the absolute nuclear DNA content and chromosome numbers and significant positive correlation between the DNA content and both cell size and number of terminal lobes. Moreover, the results showed the importance of cell/life cycle studies for interpretation of DNA content measurements in microalgae. It is now clear that whole genome duplications have occurred in all eukaryotic evolutionary lineages, and that the vast majority of flowering plants have experienced polyploidisation in their evolutionary history. However, study of genome size variation in microalgae lags behind that of higher plants and seaweeds. In this study, we have addressed the question whether microalgal phylogeny is associated with DNA content variation in order to evaluate the evolutionary significance of polyploidy in the model genus Micrasterias. We applied flow-cytometric techniques of DNA quantification to microalgae and mapped the estimated DNA content along the phylogenetic tree. Correlations between DNA content and cell morphometric parameters were also tested using geometric morphometrics. In total, DNA content was successfully determined for 34 strains of the genus Micrasterias. The estimated absolute 2C nuclear DNA amount ranged from 2.1 to 64.7 pg; intraspecific variation being 17.4-30.7 pg in M. truncata and 32.0-64.7 pg in M. rotata. There were significant differences between DNA contents of related species. We found strong correlation between the absolute nuclear DNA content and chromosome numbers and significant positive correlation between the DNA content and both cell size and number of terminal lobes. Moreover, the results showed the importance of cell/life cycle studies for interpretation of DNA content measurements in microalgae.
dcterms:title
DNA Content Variation and Its Significance in the Evolution of the Genus Micrasterias (Desmidiales, Streptophyta) DNA Content Variation and Its Significance in the Evolution of the Genus Micrasterias (Desmidiales, Streptophyta)
skos:prefLabel
DNA Content Variation and Its Significance in the Evolution of the Genus Micrasterias (Desmidiales, Streptophyta) DNA Content Variation and Its Significance in the Evolution of the Genus Micrasterias (Desmidiales, Streptophyta)
skos:notation
RIV/00216208:11310/14:10283468!RIV15-MSM-11310___
n3:aktivita
n8:S n8:P n8:I n8:V
n3:aktivity
I, P(GA206/09/0906), S, V
n3:cisloPeriodika
1
n3:dodaniDat
n4:2015
n3:domaciTvurceVysledku
n16:2548739 n16:2896729
n3:druhVysledku
n20:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
11993
n3:idVysledku
RIV/00216208:11310/14:10283468
n3:jazykVysledku
n17:eng
n3:klicovaSlova
c-value; eastern alps; green microalgae; chromosome-number; molecular phylogeny; sexual reproduction; ecological implications; flow-cytometry; genome size; nuclear-dna
n3:klicoveSlovo
n7:ecological%20implications n7:eastern%20alps n7:chromosome-number n7:flow-cytometry n7:genome%20size n7:c-value n7:sexual%20reproduction n7:green%20microalgae n7:nuclear-dna n7:molecular%20phylogeny
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[88205ECEF1AB]
n3:nazevZdroje
PLoS ONE
n3:obor
n19:EF
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
6
n3:projekt
n18:GA206%2F09%2F0906
n3:rokUplatneniVysledku
n4:2014
n3:svazekPeriodika
9
n3:tvurceVysledku
Mazalová, Petra Poulíčková, Aloisie Škaloud, Pavel Vašut, Radim J. Neustupa, Jiří Šarhanová, Petra
n3:wos
000330244500212
s:issn
1932-6203
s:numberOfPages
12
n5:doi
10.1371/journal.pone.0086247
n13:organizacniJednotka
11310