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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F14%3A10227337%21RIV15-MSM-11310___
rdf:type
n17:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1371/journal.pgen.1004092
dcterms:description
Although asexual reproduction via clonal propagation has been proposed as the principal reproductive mechanism across parasitic protozoa of the Leishmania genus, sexual recombination has long been suspected, based on hybrid marker profiles detected in field isolates from different geographical locations. The recent experimental demonstration of a sexual cycle in Leishmania within sand flies has confirmed the occurrence of hybridisation, but knowledge of the parasite life cycle in the wild still remains limited. Here, we use whole genome sequencing to investigate the frequency of sexual reproduction in Leishmania, by sequencing the genomes of 11 Leishmania infantum isolates from sand flies and 1 patient isolate in a focus of cutaneous leishmaniasis in the Cukurova province of southeast Turkey. This is the first genome-wide examination of a vector-isolated population of Leishmania parasites. A genome-wide pattern of patchy heterozygosity and SNP density was observed both within individual strains and across the whole group. Comparisons with other Leishmania donovani complex genome sequences suggest that these isolates are derived from a single cross of two diverse strains with subsequent recombination within the population. This interpretation is supported by a statistical model of the genomic variability for each strain compared to the L. infantum reference genome strain as well as genome-wide scans for recombination within the population. Further analysis of these heterozygous blocks indicates that the two parents were phylogenetically distinct. Patterns of linkage disequilibrium indicate that this population reproduced primarily clonally following the original hybridisation event, but that some recombination also occurred. This observation allowed us to estimate the relative rates of sexual and asexual reproduction within this population, to our knowledge the first quantitative estimate of these events during the Leishmania life cycle. Although asexual reproduction via clonal propagation has been proposed as the principal reproductive mechanism across parasitic protozoa of the Leishmania genus, sexual recombination has long been suspected, based on hybrid marker profiles detected in field isolates from different geographical locations. The recent experimental demonstration of a sexual cycle in Leishmania within sand flies has confirmed the occurrence of hybridisation, but knowledge of the parasite life cycle in the wild still remains limited. Here, we use whole genome sequencing to investigate the frequency of sexual reproduction in Leishmania, by sequencing the genomes of 11 Leishmania infantum isolates from sand flies and 1 patient isolate in a focus of cutaneous leishmaniasis in the Cukurova province of southeast Turkey. This is the first genome-wide examination of a vector-isolated population of Leishmania parasites. A genome-wide pattern of patchy heterozygosity and SNP density was observed both within individual strains and across the whole group. Comparisons with other Leishmania donovani complex genome sequences suggest that these isolates are derived from a single cross of two diverse strains with subsequent recombination within the population. This interpretation is supported by a statistical model of the genomic variability for each strain compared to the L. infantum reference genome strain as well as genome-wide scans for recombination within the population. Further analysis of these heterozygous blocks indicates that the two parents were phylogenetically distinct. Patterns of linkage disequilibrium indicate that this population reproduced primarily clonally following the original hybridisation event, but that some recombination also occurred. This observation allowed us to estimate the relative rates of sexual and asexual reproduction within this population, to our knowledge the first quantitative estimate of these events during the Leishmania life cycle.
dcterms:title
Genomic Confirmation of Hybridisation and Recent Inbreeding in a Vector-Isolated Leishmania Population Genomic Confirmation of Hybridisation and Recent Inbreeding in a Vector-Isolated Leishmania Population
skos:prefLabel
Genomic Confirmation of Hybridisation and Recent Inbreeding in a Vector-Isolated Leishmania Population Genomic Confirmation of Hybridisation and Recent Inbreeding in a Vector-Isolated Leishmania Population
skos:notation
RIV/00216208:11310/14:10227337!RIV15-MSM-11310___
n3:aktivita
n10:I
n3:aktivity
I
n3:cisloPeriodika
1
n3:dodaniDat
n15:2015
n3:domaciTvurceVysledku
n13:7792069 n13:6447910
n3:druhVysledku
n14:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
18150
n3:idVysledku
RIV/00216208:11310/14:10227337
n3:jazykVysledku
n6:eng
n3:klicovaSlova
donovani; infantum; life-cycle; clonal theory; genetic exchange; parasitic protozoa; cutaneous leishmaniasis; trypanosoma-brucei; visceral leishmaniasis; microsatellite typing mlmt
n3:klicoveSlovo
n4:microsatellite%20typing%20mlmt n4:genetic%20exchange n4:cutaneous%20leishmaniasis n4:clonal%20theory n4:life-cycle n4:visceral%20leishmaniasis n4:trypanosoma-brucei n4:donovani n4:parasitic%20protozoa n4:infantum
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[7E3DB53338D0]
n3:nazevZdroje
PLoS Genetics
n3:obor
n18:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
10
n3:rokUplatneniVysledku
n15:2014
n3:svazekPeriodika
10
n3:tvurceVysledku
Smith, Barbara A. Volf, Petr Smith, Deborah F. Rogers, Matthew B. Svobodová, Milena Downing, Tim Cotton, James A. Berriman, Matthew Imamura, Hideo Sanders, Mandy
n3:wos
000336525000051
s:issn
1553-7390
s:numberOfPages
14
n11:doi
10.1371/journal.pgen.1004092
n8:organizacniJednotka
11310