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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F14%3A10210464%21RIV15-MSM-11310___
rdf:type
skos:Concept n14:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.2478/s11532-014-0516-4
dcterms:description
Novel electrochemical DNA-based biosensors with outer-sphere Nafion and chitosan protective membranes were prepared for the evaluation of antioxidant properties of beverages (beer, coffee, and black tea) against prooxidant hydroxyl radicals. A carbon working electrode of a screen-printed three-electrode assembly was modified using a layer-by-layer deposition technique with low molecular weight double-stranded DNA and a Nafion or chitosan film. The membrane-covered DNA biosensors were initially tested with respect to their voltammetric and impedimetric response after the incubation of the beverage and the medium exchange for the solution of the redox indicator [Fe(CN)(6)](3-/4-). While the Nafion-protected biosensor proved to be suitable for beer and black tea extracts, the chitosan-protected biosensor was successfully used in a coffee extract. Afterwards, the applicability was successfully verified for these biosensors for the detection of a deep degradation of the surface-attached DNA at the incubation in the cleavage agent (hydroxyl radicals generated via Fenton reaction) and for the evaluation of antioxidant properties of coffee and black tea extracts against prooxidant hydroxyl radicals. The investigation of the novel biosensors with a protective membrane represents a significant contribution to the field of electrochemical DNA biosensors utilization. Novel electrochemical DNA-based biosensors with outer-sphere Nafion and chitosan protective membranes were prepared for the evaluation of antioxidant properties of beverages (beer, coffee, and black tea) against prooxidant hydroxyl radicals. A carbon working electrode of a screen-printed three-electrode assembly was modified using a layer-by-layer deposition technique with low molecular weight double-stranded DNA and a Nafion or chitosan film. The membrane-covered DNA biosensors were initially tested with respect to their voltammetric and impedimetric response after the incubation of the beverage and the medium exchange for the solution of the redox indicator [Fe(CN)(6)](3-/4-). While the Nafion-protected biosensor proved to be suitable for beer and black tea extracts, the chitosan-protected biosensor was successfully used in a coffee extract. Afterwards, the applicability was successfully verified for these biosensors for the detection of a deep degradation of the surface-attached DNA at the incubation in the cleavage agent (hydroxyl radicals generated via Fenton reaction) and for the evaluation of antioxidant properties of coffee and black tea extracts against prooxidant hydroxyl radicals. The investigation of the novel biosensors with a protective membrane represents a significant contribution to the field of electrochemical DNA biosensors utilization.
dcterms:title
DNA-based biosensors with external Nafion and chitosan membranes for the evaluation of the antioxidant activity of beer, coffee, and tea DNA-based biosensors with external Nafion and chitosan membranes for the evaluation of the antioxidant activity of beer, coffee, and tea
skos:prefLabel
DNA-based biosensors with external Nafion and chitosan membranes for the evaluation of the antioxidant activity of beer, coffee, and tea DNA-based biosensors with external Nafion and chitosan membranes for the evaluation of the antioxidant activity of beer, coffee, and tea
skos:notation
RIV/00216208:11310/14:10210464!RIV15-MSM-11310___
n3:aktivita
n10:P n10:I
n3:aktivity
I, P(GP13-23337P)
n3:cisloPeriodika
5
n3:dodaniDat
n9:2015
n3:domaciTvurceVysledku
n20:8805865
n3:druhVysledku
n11:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
12005
n3:idVysledku
RIV/00216208:11310/14:10210464
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Antioxidant properties of beverages; Reactive oxygen species; DNA damage; Outer-sphere protective membranes; Electrochemical biosensors
n3:klicoveSlovo
n7:DNA%20damage n7:Antioxidant%20properties%20of%20beverages n7:Outer-sphere%20protective%20membranes n7:Reactive%20oxygen%20species n7:Electrochemical%20biosensors
n3:kodStatuVydavatele
PL - Polská republika
n3:kontrolniKodProRIV
[A2360281342A]
n3:nazevZdroje
Central European Journal of Chemistry
n3:obor
n4:CG
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:projekt
n15:GP13-23337P
n3:rokUplatneniVysledku
n9:2014
n3:svazekPeriodika
12
n3:tvurceVysledku
Vyskočil, Vlastimil Borbelyova, Monika Hlavata, Lenka Benikova, Katarina Labuda, Jan
n3:wos
000331644400008
s:issn
1895-1066
s:numberOfPages
8
n18:doi
10.2478/s11532-014-0516-4
n16:organizacniJednotka
11310