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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F14%3A10209854%21RIV15-MSM-11310___
rdf:type
n7:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1111/j.1600-048X.2011.05860.x
dcterms:description
Researchers interested in ecological immunology face substantial methodological problems: 1) most immunological approaches are difficult to perform in free-living animals, 2) in some of the applicable methods the immunological background of the test remains unclear. The latter is also true for the PHA skin-swelling test, a trait of cell-mediated immunity commonly measured in ecology. A lack of direct evidence documenting the immunological processes in the tissue limits our understanding of the mechanism triggering the response to PHA. In our study we investigated the association between the PHA-induced skin-swelling and tissue cytokine expression in males of Perdix perdix. In PHA-challenged birds we assessed expression of nine cytokines in wing-web skin during an early stage of the immune response. We examined the relationship between the magnitude of tissue swelling and cytokine expression. We did not find any differential expression of T-cell growth factor, IL-2, in the tissue. Hence, T-cell proliferation at the time of the swelling measurement is unlikely. We detected differential expression in Th17 pro-inflammatory (IL-1β, IL-6) and anti-inflammatory (TGF-β) cytokines. The PHA-induced swelling response was only weakly linked to the expression of TGF-β. We also found relationships between the PHA-induced swelling response and phenotypic traits; the PHA swelling was positively associated with the extent of melanin-based breast ornamentation and negatively related to body size. Our results might suggest that variation in swelling is influenced by total numbers of responding cells rather than by differences in signalling. Moreover, we revealed significant correlations in expression of IL-1β, IL-6 and TGF-β. These findings are the first to show that the PHA skin-swelling test actually measures inflammation process which is part of innate immune defence and not the adaptive immune response (as assumed if the test was the reflection of T-cell proliferation). Researchers interested in ecological immunology face substantial methodological problems: 1) most immunological approaches are difficult to perform in free-living animals, 2) in some of the applicable methods the immunological background of the test remains unclear. The latter is also true for the PHA skin-swelling test, a trait of cell-mediated immunity commonly measured in ecology. A lack of direct evidence documenting the immunological processes in the tissue limits our understanding of the mechanism triggering the response to PHA. In our study we investigated the association between the PHA-induced skin-swelling and tissue cytokine expression in males of Perdix perdix. In PHA-challenged birds we assessed expression of nine cytokines in wing-web skin during an early stage of the immune response. We examined the relationship between the magnitude of tissue swelling and cytokine expression. We did not find any differential expression of T-cell growth factor, IL-2, in the tissue. Hence, T-cell proliferation at the time of the swelling measurement is unlikely. We detected differential expression in Th17 pro-inflammatory (IL-1β, IL-6) and anti-inflammatory (TGF-β) cytokines. The PHA-induced swelling response was only weakly linked to the expression of TGF-β. We also found relationships between the PHA-induced swelling response and phenotypic traits; the PHA swelling was positively associated with the extent of melanin-based breast ornamentation and negatively related to body size. Our results might suggest that variation in swelling is influenced by total numbers of responding cells rather than by differences in signalling. Moreover, we revealed significant correlations in expression of IL-1β, IL-6 and TGF-β. These findings are the first to show that the PHA skin-swelling test actually measures inflammation process which is part of innate immune defence and not the adaptive immune response (as assumed if the test was the reflection of T-cell proliferation).
dcterms:title
Cytokine expression in phytohaemagglutinin-induced skin inflammation in a galliform bird Cytokine expression in phytohaemagglutinin-induced skin inflammation in a galliform bird
skos:prefLabel
Cytokine expression in phytohaemagglutinin-induced skin inflammation in a galliform bird Cytokine expression in phytohaemagglutinin-induced skin inflammation in a galliform bird
skos:notation
RIV/00216208:11310/14:10209854!RIV15-MSM-11310___
n3:aktivita
n17:I n17:S n17:P
n3:aktivity
I, P(GA206/08/1281), P(GAP505/10/1871), P(GPP502/12/P179), S
n3:cisloPeriodika
1
n3:dodaniDat
n12:2015
n3:domaciTvurceVysledku
n10:9248870 n10:2571544 n10:9557342
n3:druhVysledku
n20:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
9546
n3:idVysledku
RIV/00216208:11310/14:10209854
n3:jazykVysledku
n4:eng
n3:klicovaSlova
mechanisms; coloration; immunology; immunocompetence; house sparrows; passer-domesticus; absolute quantification; perdix-perdix; grey partridge; cellular immune-response
n3:klicoveSlovo
n8:absolute%20quantification n8:house%20sparrows n8:immunology n8:cellular%20immune-response n8:mechanisms n8:immunocompetence n8:grey%20partridge n8:passer-domesticus n8:perdix-perdix n8:coloration
n3:kodStatuVydavatele
DK - Dánské království
n3:kontrolniKodProRIV
[C54D06645ECA]
n3:nazevZdroje
Journal of Avian Biology
n3:obor
n15:EG
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
5
n3:projekt
n11:GPP502%2F12%2FP179 n11:GAP505%2F10%2F1871 n11:GA206%2F08%2F1281
n3:rokUplatneniVysledku
n12:2014
n3:svazekPeriodika
45
n3:tvurceVysledku
Vinkler, Michal Gabrielová, Barbora Bryjova, Anna Bainová, Hana Svobodova, Jana
n3:wos
000329866900007
s:issn
0908-8857
s:numberOfPages
8
n9:doi
10.1111/j.1600-048X.2011.05860.x
n18:organizacniJednotka
11310